Title

The Effects Of High Fat Diet Induced Obesity On The Translational Capacity And Efficiency In The Skeletal Muscle Of Mice

Author(s)

Nathan Pignone

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: James A. Carson, Department of Exercise Science, University of South Carolina

Abstract

Obesity is an extremely common and rapidly growing medical conditions and is often a result of an unhealthy diet. Skeletal muscle is important for a healthy metabolism and regular function. Therefore, it is a fitting study model for muscular effects of obesity which disrupt the metabolic balance of protein turnover. Specifically, this study will focus on how obesity impacts translational capacity and efficiency. The purpose is to better understand what occurs metabolically within the skeletal muscles. For this experiment, three treatments of C57BL/6 mice were used. Two groups (3 and 18 months old) were fed a standard chow diet (n=10 each), the final group was 18 months old and fed a high-fat diet (n=10). The right quadriceps were tested for protein and RNA content and concentration to determine translation capacity and efficiency. Both the 18 month chow and high-fat diet (HFD) groups had significantly higher body weights from the 3 month chow treatment at sacrifice with HFD group also being significantly higher than the 18 month chow group. The 18 month chow sacrifice muscle weights were the only group that displayed significance to the younger control, (set at 0.05) despite the gap in total body weight between the HFD group and the other two groups. Relative muscle weight to body weight comparisons low muscle mass in the HFD mice. HFD mice also demonstrated severe declines in both RNA and protein concentration and content. All groups lived similar lives, so the diet is most likely to have caused these differences.

Start Date

4-11-2015 2:00 PM

End Date

4-11-2015 2:15 PM

COinS
 
Apr 11th, 2:00 PM Apr 11th, 2:15 PM

The Effects Of High Fat Diet Induced Obesity On The Translational Capacity And Efficiency In The Skeletal Muscle Of Mice

Obesity is an extremely common and rapidly growing medical conditions and is often a result of an unhealthy diet. Skeletal muscle is important for a healthy metabolism and regular function. Therefore, it is a fitting study model for muscular effects of obesity which disrupt the metabolic balance of protein turnover. Specifically, this study will focus on how obesity impacts translational capacity and efficiency. The purpose is to better understand what occurs metabolically within the skeletal muscles. For this experiment, three treatments of C57BL/6 mice were used. Two groups (3 and 18 months old) were fed a standard chow diet (n=10 each), the final group was 18 months old and fed a high-fat diet (n=10). The right quadriceps were tested for protein and RNA content and concentration to determine translation capacity and efficiency. Both the 18 month chow and high-fat diet (HFD) groups had significantly higher body weights from the 3 month chow treatment at sacrifice with HFD group also being significantly higher than the 18 month chow group. The 18 month chow sacrifice muscle weights were the only group that displayed significance to the younger control, (set at 0.05) despite the gap in total body weight between the HFD group and the other two groups. Relative muscle weight to body weight comparisons low muscle mass in the HFD mice. HFD mice also demonstrated severe declines in both RNA and protein concentration and content. All groups lived similar lives, so the diet is most likely to have caused these differences.