Utilizing Adventitious Root Variattion To Identify The Correlation Between Root Formation And Leaves In Peaches
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Botany
Presentation Type
Mentored
Abstract
Adventitious rooting of plants is beneficial to the cloning of desired plants; no matter the use. However, many desired species of plants do not produce adventitious roots, so the characteristics of adventitious roots are studied to see if these desired species of plant can get the genome needed to do so. In this research, the ultimate goal is to identify the different that leaves make when growing adventitious roots. Two populations, A and C, are phenotyped for their production of adventitious roots. Approximately ten, twenty-centimeter cuttings of current year wood were taken from 378 trees in the A population and 57 trees in the C population. The leaves were stripped and the stems were dipped in IBA, indole-3-butyric acid, and placed in a soilless media, which consisted of vermiculite and perlite. They were then put in the greenhouse and kept misted for four weeks. The cuttings were scored for various properties such as: number dead, callus, rooting, and budding. DNA from the dried leaf tissues of the same populations were extracted quantified and quality checked in order to produce a more detailed genetic map. A second experiment was done after this with the same populations leaving the leaves on the cuttings to see if they make a difference. The success of the growing stems, with or without leaves, will tell us whether the rooting of the plants can be controlled so that maybe in the future the genome of adventitious rooting can be modified and placed in other plants.
Recommended Citation
Cornish, TK, "Utilizing Adventitious Root Variattion To Identify The Correlation Between Root Formation And Leaves In Peaches" (2015). South Carolina Junior Academy of Science. 123.
https://scholarexchange.furman.edu/scjas/2015/all/123
Start Date
4-11-2015 9:30 AM
End Date
4-11-2015 9:45 AM
Utilizing Adventitious Root Variattion To Identify The Correlation Between Root Formation And Leaves In Peaches
Adventitious rooting of plants is beneficial to the cloning of desired plants; no matter the use. However, many desired species of plants do not produce adventitious roots, so the characteristics of adventitious roots are studied to see if these desired species of plant can get the genome needed to do so. In this research, the ultimate goal is to identify the different that leaves make when growing adventitious roots. Two populations, A and C, are phenotyped for their production of adventitious roots. Approximately ten, twenty-centimeter cuttings of current year wood were taken from 378 trees in the A population and 57 trees in the C population. The leaves were stripped and the stems were dipped in IBA, indole-3-butyric acid, and placed in a soilless media, which consisted of vermiculite and perlite. They were then put in the greenhouse and kept misted for four weeks. The cuttings were scored for various properties such as: number dead, callus, rooting, and budding. DNA from the dried leaf tissues of the same populations were extracted quantified and quality checked in order to produce a more detailed genetic map. A second experiment was done after this with the same populations leaving the leaves on the cuttings to see if they make a difference. The success of the growing stems, with or without leaves, will tell us whether the rooting of the plants can be controlled so that maybe in the future the genome of adventitious rooting can be modified and placed in other plants.
Mentor
Mentor: Douglas Bielenberg, College of Agriculture, Forestry, and Life Sciences, Clemson University