Title

Utilizing Adventitious Root Variattion To Identify The Correlation Between Root Formation And Leaves In Peaches

Author(s)

TK Cornish

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Botany

Presentation Type

Mentored

Mentor

Mentor: Douglas Bielenberg, College of Agriculture, Forestry, and Life Sciences, Clemson University

Abstract

Adventitious rooting of plants is beneficial to the cloning of desired plants; no matter the use. However, many desired species of plants do not produce adventitious roots, so the characteristics of adventitious roots are studied to see if these desired species of plant can get the genome needed to do so. In this research, the ultimate goal is to identify the different that leaves make when growing adventitious roots. Two populations, A and C, are phenotyped for their production of adventitious roots. Approximately ten, twenty-centimeter cuttings of current year wood were taken from 378 trees in the A population and 57 trees in the C population. The leaves were stripped and the stems were dipped in IBA, indole-3-butyric acid, and placed in a soilless media, which consisted of vermiculite and perlite. They were then put in the greenhouse and kept misted for four weeks. The cuttings were scored for various properties such as: number dead, callus, rooting, and budding. DNA from the dried leaf tissues of the same populations were extracted quantified and quality checked in order to produce a more detailed genetic map. A second experiment was done after this with the same populations leaving the leaves on the cuttings to see if they make a difference. The success of the growing stems, with or without leaves, will tell us whether the rooting of the plants can be controlled so that maybe in the future the genome of adventitious rooting can be modified and placed in other plants.

Start Date

4-11-2015 9:30 AM

End Date

4-11-2015 9:45 AM

COinS
 
Apr 11th, 9:30 AM Apr 11th, 9:45 AM

Utilizing Adventitious Root Variattion To Identify The Correlation Between Root Formation And Leaves In Peaches

Adventitious rooting of plants is beneficial to the cloning of desired plants; no matter the use. However, many desired species of plants do not produce adventitious roots, so the characteristics of adventitious roots are studied to see if these desired species of plant can get the genome needed to do so. In this research, the ultimate goal is to identify the different that leaves make when growing adventitious roots. Two populations, A and C, are phenotyped for their production of adventitious roots. Approximately ten, twenty-centimeter cuttings of current year wood were taken from 378 trees in the A population and 57 trees in the C population. The leaves were stripped and the stems were dipped in IBA, indole-3-butyric acid, and placed in a soilless media, which consisted of vermiculite and perlite. They were then put in the greenhouse and kept misted for four weeks. The cuttings were scored for various properties such as: number dead, callus, rooting, and budding. DNA from the dried leaf tissues of the same populations were extracted quantified and quality checked in order to produce a more detailed genetic map. A second experiment was done after this with the same populations leaving the leaves on the cuttings to see if they make a difference. The success of the growing stems, with or without leaves, will tell us whether the rooting of the plants can be controlled so that maybe in the future the genome of adventitious rooting can be modified and placed in other plants.