Articular Cartilage And Its Potentail For Regeneration When Grown Using A Novel Technique For Culturing

Joshua Pope

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Mentor

Mentor: Delphine Dean, Department of Bioengineering, Clemson University

Abstract

Articular cartilage is found inside the rotator joints of the body. The articular cartilage acts as a sort of cushion for the joints during movement in order to prevent friction. During a normal life the articular cartilage is subjected to a substantial amount of damage leading to a multitude of issues concerning the rotating joints. Chondrocytes, which are the cells that make up the articular cartilage, were grown using the hanging drop technique while suspended in both regular chondrocyte media and media supplemented with methylcellulose. The hanging drop technique should create 3-D balls of chondroctyes known as spheroids with augmented regenerative potential. The objective of this research was to observe the effect that growing the spheroids in supplemented media has on the properties of the spheroids being produced.

Start Date

4-11-2015 11:15 AM

End Date

4-11-2015 11:30 AM

COinS
 
Apr 11th, 11:15 AM Apr 11th, 11:30 AM

Articular Cartilage And Its Potentail For Regeneration When Grown Using A Novel Technique For Culturing

Articular cartilage is found inside the rotator joints of the body. The articular cartilage acts as a sort of cushion for the joints during movement in order to prevent friction. During a normal life the articular cartilage is subjected to a substantial amount of damage leading to a multitude of issues concerning the rotating joints. Chondrocytes, which are the cells that make up the articular cartilage, were grown using the hanging drop technique while suspended in both regular chondrocyte media and media supplemented with methylcellulose. The hanging drop technique should create 3-D balls of chondroctyes known as spheroids with augmented regenerative potential. The objective of this research was to observe the effect that growing the spheroids in supplemented media has on the properties of the spheroids being produced.