The Effect Of Chitosan Solutions On The Reproduction Of Evolving Pathogen And Nosocomial Infection Staphylococcus Epidermidis

Alyssia Mitchell

Abstract

Staphylococcus epidermidis is an evolving pathogen and is antibiotic resistant. The purpose of this experiment was to find a cost efficient cure for the evolving pathogen and nosocomial infection Staphylococcus epidermidis. Chitosan is a derivative of the partial deacetylation of chitin and it is a natural polysaccharide. It is a biodegradable and biocompatible substance. It was hypothesized that if Staphylococcus epidermidis is treated by a chitosan solution A then Staphylococcus epidermidis will have a lower optical density than if it was treated with a chitosan solution B. The method of conducting the experiment was inoculating Staphylococcus epidermidis into test tubes of sterilized and preordered nutrient broth. They were then labeled and placed into an incubator for 48 hours at 37° C. After 48 hours, chitosan solution was then added to the test tubes. The test tubes were then placed back into the incubator for 48 hours at 37° C. Once the test tubes were removed, the optical density of each tube was taken at 396.4 nm and recorded. The control for this experiment was Staphylococcus epidermidis inoculated in nutrient broth using aseptic technique. The data was analyzed using a One-Way ANOVA at α=.05. There was enough evidence to reject the null hypothesis, F(2,37)= 8.3784, p<.001, so a post-hoc Scheffé test was run. It was then concluded that the hypothesis was supported and chitosan solutions are successful inhibiting the growth of Staphylococcus epidermidis.

 
Apr 10th, 12:00 AM

The Effect Of Chitosan Solutions On The Reproduction Of Evolving Pathogen And Nosocomial Infection Staphylococcus Epidermidis

Staphylococcus epidermidis is an evolving pathogen and is antibiotic resistant. The purpose of this experiment was to find a cost efficient cure for the evolving pathogen and nosocomial infection Staphylococcus epidermidis. Chitosan is a derivative of the partial deacetylation of chitin and it is a natural polysaccharide. It is a biodegradable and biocompatible substance. It was hypothesized that if Staphylococcus epidermidis is treated by a chitosan solution A then Staphylococcus epidermidis will have a lower optical density than if it was treated with a chitosan solution B. The method of conducting the experiment was inoculating Staphylococcus epidermidis into test tubes of sterilized and preordered nutrient broth. They were then labeled and placed into an incubator for 48 hours at 37° C. After 48 hours, chitosan solution was then added to the test tubes. The test tubes were then placed back into the incubator for 48 hours at 37° C. Once the test tubes were removed, the optical density of each tube was taken at 396.4 nm and recorded. The control for this experiment was Staphylococcus epidermidis inoculated in nutrient broth using aseptic technique. The data was analyzed using a One-Way ANOVA at α=.05. There was enough evidence to reject the null hypothesis, F(2,37)= 8.3784, p<.001, so a post-hoc Scheffé test was run. It was then concluded that the hypothesis was supported and chitosan solutions are successful inhibiting the growth of Staphylococcus epidermidis.