A 3D Microscope For Visualization Of Thick Samples
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Engineering
Presentation Type
Mentored
Oral Presentation Award
3rd Place
Abstract
Three dimensional images of cells can be produced by multiphoton excitation and confocal laser scanning microscopy, in which a laser scans the cell point-by-point and layer-by-layer. Though the lateral scan is executed rapidly (several kHz), scanning through the entire depth of thick samples hinders the speed of real-time visualization of the volume. Here we use a different type of laser known as a Bessel beam, which does not diffract based on its unique properties. The beam’s extended focus eliminates the need to scan the depth, allowing the entire volume of the cell to be recorded by a lateral scan of the sample. Recording the volume from two different angles, known as a parallax view, provides the sample’s depth information. This pair of images can be combined using computer code to create a red-cyan stereoscopic image of the volume. These stereo images can be viewed in as a real-time three dimensional scan of a sample.
Recommended Citation
Maes, Cameron, "A 3D Microscope For Visualization Of Thick Samples" (2015). South Carolina Junior Academy of Science. 3.
https://scholarexchange.furman.edu/scjas/2015/all/3
Start Date
4-11-2015 8:30 AM
End Date
4-11-2015 8:45 AM
A 3D Microscope For Visualization Of Thick Samples
Three dimensional images of cells can be produced by multiphoton excitation and confocal laser scanning microscopy, in which a laser scans the cell point-by-point and layer-by-layer. Though the lateral scan is executed rapidly (several kHz), scanning through the entire depth of thick samples hinders the speed of real-time visualization of the volume. Here we use a different type of laser known as a Bessel beam, which does not diffract based on its unique properties. The beam’s extended focus eliminates the need to scan the depth, allowing the entire volume of the cell to be recorded by a lateral scan of the sample. Recording the volume from two different angles, known as a parallax view, provides the sample’s depth information. This pair of images can be combined using computer code to create a red-cyan stereoscopic image of the volume. These stereo images can be viewed in as a real-time three dimensional scan of a sample.
