Title

High Speed 3D Imaging With Two-Photon Light Sheet Microscopy

School Name

Academic Magnet High School

Grade Level

12th Grade

Presentation Topic

Engineering

Presentation Type

Mentored

Mentor

Mentor: Dr. Tong Ye; Clemson/MUSC

Abstract

The current image speed of multi-photon microscopy is not enough for fast biological processes such as molecular diffusion, so improvements were made to existing optical designs. Two-photon microscopy and light sheet fluorescent microscopy were combined and used to create the two-photon light sheet microscope which offers optical sectioning at a much faster speed than previous designs. The capabilities of the microscope were used to perform the first three-dimensional Fluorescent Recovery After Photobleaching experiment, as well as image cardiomyocyte spheroid cells. The microscope is shown to be the fastest method for imaging fast biological processes in three-dimensions.

Location

Owens G07

Start Date

4-16-2016 12:00 PM

COinS
 
Apr 16th, 12:00 PM

High Speed 3D Imaging With Two-Photon Light Sheet Microscopy

Owens G07

The current image speed of multi-photon microscopy is not enough for fast biological processes such as molecular diffusion, so improvements were made to existing optical designs. Two-photon microscopy and light sheet fluorescent microscopy were combined and used to create the two-photon light sheet microscope which offers optical sectioning at a much faster speed than previous designs. The capabilities of the microscope were used to perform the first three-dimensional Fluorescent Recovery After Photobleaching experiment, as well as image cardiomyocyte spheroid cells. The microscope is shown to be the fastest method for imaging fast biological processes in three-dimensions.