Cell-Cell Interactions Of Human Umbilical Vein Endothelial Cells (Huvec) And Aortic Smooth Muscle Cells On Cultispher Bead Scaffolding

Dhruw Maisuria

School Name

Governor's School for Science and Math

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Mentor

Mentor: Dr. Hammad; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina

Abstract

The human population has started to live for longer periods of time indicating that there might be a need for donor organs for transplant. Due to the limitations in the number of organs, patients are unable to survive and also there is the risk of immune rejection of a donated organ. This can be avoided if the patients own cells can be used to make the organs. The major aim of the project is to ultimately be able to produce artificial blood vessels for humans as well as to determine a technique by which blood vessels can be surgically implanted into humans. The aim of this research is to generate preliminary data on cell-cell interactions in blood vessels. The cells used in this research are Aortic smooth muscle and Human Umbilical Vein Endothelial (HUVEC) cells. The cells were incubated with gelatinous macro porous spheres called cultisphers, as they were the best material to grow cells. The cells were grown independently in Fetal Bovine Serum (FBS) media for two days, and they were then mixed together into wells and were incubated for a total of nine days. The cells were then stained with Cell Tracker CMFDA and observed under confocal microscopy and bright field microscopy. The resulting images showed that when cells were grown together they had web-like cells growth in the areas between the beads and connected the beads together as compared to cells grown independently, but this was not confirmed, by confocal microscopy as the stain antibody was ineffective.

Location

Owens 107

Start Date

4-16-2016 9:30 AM

COinS
 
Apr 16th, 9:30 AM

Cell-Cell Interactions Of Human Umbilical Vein Endothelial Cells (Huvec) And Aortic Smooth Muscle Cells On Cultispher Bead Scaffolding

Owens 107

The human population has started to live for longer periods of time indicating that there might be a need for donor organs for transplant. Due to the limitations in the number of organs, patients are unable to survive and also there is the risk of immune rejection of a donated organ. This can be avoided if the patients own cells can be used to make the organs. The major aim of the project is to ultimately be able to produce artificial blood vessels for humans as well as to determine a technique by which blood vessels can be surgically implanted into humans. The aim of this research is to generate preliminary data on cell-cell interactions in blood vessels. The cells used in this research are Aortic smooth muscle and Human Umbilical Vein Endothelial (HUVEC) cells. The cells were incubated with gelatinous macro porous spheres called cultisphers, as they were the best material to grow cells. The cells were grown independently in Fetal Bovine Serum (FBS) media for two days, and they were then mixed together into wells and were incubated for a total of nine days. The cells were then stained with Cell Tracker CMFDA and observed under confocal microscopy and bright field microscopy. The resulting images showed that when cells were grown together they had web-like cells growth in the areas between the beads and connected the beads together as compared to cells grown independently, but this was not confirmed, by confocal microscopy as the stain antibody was ineffective.