Title

The Effect Of Time Treatment Of Panax Quinquefolius On Nrf2 And Nqo1 Levels In Ana-1 Cells

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

The effect of time treatment of Panax quinquefolius on Nrf2 and NQO1 levels in ANA-1 cells / Shakthika Saravanan / Medical Sciences / / Around 93,090 cases and 49,700 deaths, resulting from colorectal cancer, were estimated for 2015 (“Cancer facts and figures 2015,” 2015). This study focused on ulcerative colitis induced colon cancer which are promoted through inflammation. The purpose of the study was to increase the Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 (NQO1) production in non-carcinogenic cells to help prevent and protect the cells from inflammation. It was hypothesized that Panax quinquefolius (american ginseng) could accomplish this. ANA-1 cells were treated with 250 ug/mL of american ginseng diluted with Dulbecco's modified Eagle's media (DMEM) with 0.1% fetal bovine serum (FBS) at a two hour interval beginning at 10:00 pm. At 10:00 pm, all the plates were cleansed. Several procedures were completed to prepare the samples for the western blot procedure. The bands of proteins were identified and exposed afterwards. The bands revealed that the Nrf2 increased significantly. NQO1 also increased a significant amount, but decreased after 6 hours of treatment. Hence, it was derived that the Nrf2, if under oxidative stress, would combat the oxidative species in the cell. However, it was also plausible that the great amount of Nrf2 could not reach the DNA. Thus, the NQO1 and other possible downstream proteins could not be activated. /

Location

Owens 202

Start Date

4-16-2016 10:30 AM

COinS
 
Apr 16th, 10:30 AM

The Effect Of Time Treatment Of Panax Quinquefolius On Nrf2 And Nqo1 Levels In Ana-1 Cells

Owens 202

The effect of time treatment of Panax quinquefolius on Nrf2 and NQO1 levels in ANA-1 cells / Shakthika Saravanan / Medical Sciences / / Around 93,090 cases and 49,700 deaths, resulting from colorectal cancer, were estimated for 2015 (“Cancer facts and figures 2015,” 2015). This study focused on ulcerative colitis induced colon cancer which are promoted through inflammation. The purpose of the study was to increase the Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 (NQO1) production in non-carcinogenic cells to help prevent and protect the cells from inflammation. It was hypothesized that Panax quinquefolius (american ginseng) could accomplish this. ANA-1 cells were treated with 250 ug/mL of american ginseng diluted with Dulbecco's modified Eagle's media (DMEM) with 0.1% fetal bovine serum (FBS) at a two hour interval beginning at 10:00 pm. At 10:00 pm, all the plates were cleansed. Several procedures were completed to prepare the samples for the western blot procedure. The bands of proteins were identified and exposed afterwards. The bands revealed that the Nrf2 increased significantly. NQO1 also increased a significant amount, but decreased after 6 hours of treatment. Hence, it was derived that the Nrf2, if under oxidative stress, would combat the oxidative species in the cell. However, it was also plausible that the great amount of Nrf2 could not reach the DNA. Thus, the NQO1 and other possible downstream proteins could not be activated. /