Title

Quantifying Erythrocyte Carbohydrate Surface Antigen Concentration In Lipid Rafts

Author(s)

Matthew Heron

School Name

Center for Advanced Technical Studies

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Non-Mentored

Oral Presentation Award

1st Place

Abstract

The lack of sufficient blood stores is a major problem facing modern medicine. In the United States alone, blood transfusion volume sums to roughly thirty million pints of blood being utilized each year. A complication thus arises in maintaining sufficient blood stores to meet this growing demand. To exacerbate an already precarious condition, blood types further attenuate the supply of blood in proportion to those who need it. The purpose of this project is to aide current research targeted at neutralizing blood antigens. Since surface antigens are carbohydrate molecules that bind to glycolipids and glycoproteins, it is hypothesized that surface antigens will be concentrated on or around lipid rafts in the cell membrane of erythrocytes where there are increased concentrations of sphingolipids. Lipid rafts will be isolated from the plasma membrane using serial buffer dilution. Concentration of N-acetylgalactosamine and galactose will then be measured using biotinylated wisteria floribunda lectins in biotin concentration assay. Statistical computations will determine if there is a significantly greater concentration of antigens in lipid rafts.

Location

Owens 202

Start Date

4-16-2016 10:45 AM

COinS
 
Apr 16th, 10:45 AM

Quantifying Erythrocyte Carbohydrate Surface Antigen Concentration In Lipid Rafts

Owens 202

The lack of sufficient blood stores is a major problem facing modern medicine. In the United States alone, blood transfusion volume sums to roughly thirty million pints of blood being utilized each year. A complication thus arises in maintaining sufficient blood stores to meet this growing demand. To exacerbate an already precarious condition, blood types further attenuate the supply of blood in proportion to those who need it. The purpose of this project is to aide current research targeted at neutralizing blood antigens. Since surface antigens are carbohydrate molecules that bind to glycolipids and glycoproteins, it is hypothesized that surface antigens will be concentrated on or around lipid rafts in the cell membrane of erythrocytes where there are increased concentrations of sphingolipids. Lipid rafts will be isolated from the plasma membrane using serial buffer dilution. Concentration of N-acetylgalactosamine and galactose will then be measured using biotinylated wisteria floribunda lectins in biotin concentration assay. Statistical computations will determine if there is a significantly greater concentration of antigens in lipid rafts.