Title

The Lc50'S For Nitrate And Sulfate

Author(s)

Noah Mervak

School Name

Chapin High School

Grade Level

11th Grade

Presentation Topic

Biochemistry

Presentation Type

Non-Mentored

Abstract

The goal of this project was to find the LC50 (lethal concentration required to kill 50% of the animals) for nitrate and sulfate for ceriodaphnia dubia (water flea). This was done by performing 8 acute toxicity tests; 5 conducted with NaNO3 (sodium nitrate) in dilution water, and 3 conducted with CaSO4 (calcium sulfate) in dilution water. Data yielded by NaNO3 acute toxicity tests were considered conclusive when a concentration of NaNO3 killed less than 50% of the water fleas (ceriodaphnia dubia) and a concentration of NaNO3 killed greater than 50% of the water fleas. Data yielded by CaSO4 acute toxicity tests were considered conclusive when a concentration of CaSO4 killed less than 50% of the water fleas and a concentration of CaSO4 killed greater than 50% of the water fleas. According to these standards, 2 of the 5 NaNO3 acute toxicity tests were conclusive and 2 of the 3 CaSO4 acute toxicity tests were conclusive. Using the acute toxicity tests considered to have conclusive data, 2 graphs were made, one for NaNO3 and one for CaSO4. Based on the 2 graphs made, the LC50 for NaNO3 was calculated as 3.05g/L and the LC50 for CaSO3 was calculated as 5.95g/L. Based on the LC50s for NaNO3 (3.05g/L) and CaSO4 (5.95g/L), the LC50 for nitrate was calculated as 2.22467g/L and the LC50 for sulfate was calculated as 4.19832g/L.

Location

Owens 204

Start Date

4-16-2016 9:45 AM

COinS
 
Apr 16th, 9:45 AM

The Lc50'S For Nitrate And Sulfate

Owens 204

The goal of this project was to find the LC50 (lethal concentration required to kill 50% of the animals) for nitrate and sulfate for ceriodaphnia dubia (water flea). This was done by performing 8 acute toxicity tests; 5 conducted with NaNO3 (sodium nitrate) in dilution water, and 3 conducted with CaSO4 (calcium sulfate) in dilution water. Data yielded by NaNO3 acute toxicity tests were considered conclusive when a concentration of NaNO3 killed less than 50% of the water fleas (ceriodaphnia dubia) and a concentration of NaNO3 killed greater than 50% of the water fleas. Data yielded by CaSO4 acute toxicity tests were considered conclusive when a concentration of CaSO4 killed less than 50% of the water fleas and a concentration of CaSO4 killed greater than 50% of the water fleas. According to these standards, 2 of the 5 NaNO3 acute toxicity tests were conclusive and 2 of the 3 CaSO4 acute toxicity tests were conclusive. Using the acute toxicity tests considered to have conclusive data, 2 graphs were made, one for NaNO3 and one for CaSO4. Based on the 2 graphs made, the LC50 for NaNO3 was calculated as 3.05g/L and the LC50 for CaSO3 was calculated as 5.95g/L. Based on the LC50s for NaNO3 (3.05g/L) and CaSO4 (5.95g/L), the LC50 for nitrate was calculated as 2.22467g/L and the LC50 for sulfate was calculated as 4.19832g/L.