Title

The Effect Of Hydrogen-Peroxide On Enzyme Activity In Swine Blood Measured By The Amount Of Oxygen Present

Author(s)

Leila Barwick

School Name

Heathwood Hall Episcopal School

Grade Level

11th Grade

Presentation Topic

Biochemistry

Presentation Type

Non-Mentored

Written Paper Award

2nd Place

Abstract

Throughout the world today, many people use Hydrogen Peroxide as a disinfectant for lacerations, but many people are unaware of how this commonly used solution affects your blood. The purpose of this study was to investigate the effect of Hydrogen Peroxide (H2O2) on oxygen levels in blood. The hypothesis is when hydrogen-peroxide is added to the swine blood, the enzyme activity will change, which will be shown by an increase or a decrease in the amount of O2 in the swine blood. The null hypothesis is when the hydrogen-peroxide is added to the swine blood, the enzyme activity will not change, which will be shown by a consistent O2 level in the blood. In order to keep this study ethical, swine blood was used. 50 mL of blood was poured into a 250 mL beaker, the blood was then stirred with the Oxygen sensor, which measured the amount of dissolved oxygen in ppm, for 180 seconds to collect the control group data. Then the beakers were divided into 3 groups. Next, one beaker from each group was chosen, one 1 mL, another 2mL, and lastly 3 mL of Hydrogen Peroxide. Each solution was stirred for 15 seconds before adding the hydrogen peroxide while continuously stirring until the 180 seconds ended. The results of this study showed an increase in oxygen levels of at least 1 ppm for each mL added. The conclusion of this study is that the hypothesis can be accepted, and the null hypothesis can be rejected.

Location

Owens 204

Start Date

4-16-2016 9:00 AM

COinS
 
Apr 16th, 9:00 AM

The Effect Of Hydrogen-Peroxide On Enzyme Activity In Swine Blood Measured By The Amount Of Oxygen Present

Owens 204

Throughout the world today, many people use Hydrogen Peroxide as a disinfectant for lacerations, but many people are unaware of how this commonly used solution affects your blood. The purpose of this study was to investigate the effect of Hydrogen Peroxide (H2O2) on oxygen levels in blood. The hypothesis is when hydrogen-peroxide is added to the swine blood, the enzyme activity will change, which will be shown by an increase or a decrease in the amount of O2 in the swine blood. The null hypothesis is when the hydrogen-peroxide is added to the swine blood, the enzyme activity will not change, which will be shown by a consistent O2 level in the blood. In order to keep this study ethical, swine blood was used. 50 mL of blood was poured into a 250 mL beaker, the blood was then stirred with the Oxygen sensor, which measured the amount of dissolved oxygen in ppm, for 180 seconds to collect the control group data. Then the beakers were divided into 3 groups. Next, one beaker from each group was chosen, one 1 mL, another 2mL, and lastly 3 mL of Hydrogen Peroxide. Each solution was stirred for 15 seconds before adding the hydrogen peroxide while continuously stirring until the 180 seconds ended. The results of this study showed an increase in oxygen levels of at least 1 ppm for each mL added. The conclusion of this study is that the hypothesis can be accepted, and the null hypothesis can be rejected.