Title

Effect Of Time In A Microwave On Micrococcus Luteus

School Name

Heathwood Hall Episcopal School

Grade Level

9th Grade

Presentation Topic

Consumer Science

Presentation Type

Non-Mentored

Abstract

This experiment studied the effect of time in a microwave on growth of Micrococcus luteus. Micrococcus luteus was introduced onto pieces of turkey to be microwaved (1350 W). The hypothesis is that the turkey in the microwaved for 0 seconds would have more growth than on the 25 second turkey slices did. These pieces of turkey were cut into congruent circles. The bacterial broth was mixed with two 125 mL bottles of nutrient broth to increase the amount of bacteria that was used. The broths incubated for two days in 25ºC. The slices of turkey were fully submerged in the bacterial broth. The new infected slices of turkey were microwaved for four different times with four slices for each time. These times were 10 seconds, 15 seconds, 20 seconds, 25 seconds, and 0 seconds. The petri dishes were introduced to the bacteria from the turkey using an inoculation loop. The petri dishes incubated for four days at 25ºC. The colonies were hand counted. The petri dishes that had been inoculated with turkey that were microwaved for 0 seconds have colony counts of 8, 5, 100, and 163. The 10 second dishes’ counts are 4,2,1, and 1. The 25 second dishes’ counts are 0,0,1,1. The 20 second dishes’ counts are 0, 0, 2, and 1. The 25 second dishes’ counts are 0, 1, 3, and 3. These results show that the shorter times (10 seconds) has more bacterial growth than the longer times (20 seconds, 25 seconds).

Location

Owens 103

Start Date

4-16-2016 10:00 AM

COinS
 
Apr 16th, 10:00 AM

Effect Of Time In A Microwave On Micrococcus Luteus

Owens 103

This experiment studied the effect of time in a microwave on growth of Micrococcus luteus. Micrococcus luteus was introduced onto pieces of turkey to be microwaved (1350 W). The hypothesis is that the turkey in the microwaved for 0 seconds would have more growth than on the 25 second turkey slices did. These pieces of turkey were cut into congruent circles. The bacterial broth was mixed with two 125 mL bottles of nutrient broth to increase the amount of bacteria that was used. The broths incubated for two days in 25ºC. The slices of turkey were fully submerged in the bacterial broth. The new infected slices of turkey were microwaved for four different times with four slices for each time. These times were 10 seconds, 15 seconds, 20 seconds, 25 seconds, and 0 seconds. The petri dishes were introduced to the bacteria from the turkey using an inoculation loop. The petri dishes incubated for four days at 25ºC. The colonies were hand counted. The petri dishes that had been inoculated with turkey that were microwaved for 0 seconds have colony counts of 8, 5, 100, and 163. The 10 second dishes’ counts are 4,2,1, and 1. The 25 second dishes’ counts are 0,0,1,1. The 20 second dishes’ counts are 0, 0, 2, and 1. The 25 second dishes’ counts are 0, 1, 3, and 3. These results show that the shorter times (10 seconds) has more bacterial growth than the longer times (20 seconds, 25 seconds).