Title

The Effect Of Trisprintec And Metformin And Their Doses On The Mortality And Reproduction Of Ampullariidae, Daphnia Magna, And Lemnoideae & The Heart Rate Of Daphnia Magna In Aquatic Ecosystems

Author(s)

Sarayu Das

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Environmental Science

Presentation Type

Non-Mentored

Written Paper Award

1st Place

Abstract

In recent years, an increasing level of pharmaceuticals has been detected in rivers and streams. The purpose of this experiment was to test the effect of two of the most common drugs found at the Congaree National Park in South Carolina, metformin and tri-sprintec, on three prevalent aquatic species, snails (Ampullariidae), plants (Lemnoideae), and fleas (Daphnia magna). This experiment was done in two stages: phase one to study the effect of pharmaceuticals on the organisms and phase two to find the maximum threshold of pharmaceuticals affecting the lifespan of these species. It was hypothesized that when the dosage of pharmaceuticals increased, the Daphnia magna would experience increased heart rate, and the mortality and reproduction of these organisms would be negatively affected. This was achieved by placing the organisms in separate habitats, and three different doses of tri-sprintec and metformin were gradually added to their environments over three weeks. An ANOVA test of F(2,8)=15.88, p=0.002 for the heart rate of Daphnia magna and a linear regression t-test of R(3)=0.97, p<0.01 for the natural increase rate (NIR) of Daphnia magna showed that the pharmaceuticals did have a significant impact on these organisms. Furthermore, a linear regression t-test (R(3)=0.88, p<0.04) was run for Lemnoideae with metformin, and indicated that increased doses of pharmaceuticals did have a greater impact on the organisms. A brochure was circulated throughout neighborhoods to bring awareness about the best practices of disposing unused pharmaceuticals. This community outreach was a success and the researcher plans to continue conducting classes to the public and achieving the full potential of this experiment, thus reducing this environmental problem at the source. /

Location

Owens 208

Start Date

4-16-2016 11:00 AM

COinS
 
Apr 16th, 11:00 AM

The Effect Of Trisprintec And Metformin And Their Doses On The Mortality And Reproduction Of Ampullariidae, Daphnia Magna, And Lemnoideae & The Heart Rate Of Daphnia Magna In Aquatic Ecosystems

Owens 208

In recent years, an increasing level of pharmaceuticals has been detected in rivers and streams. The purpose of this experiment was to test the effect of two of the most common drugs found at the Congaree National Park in South Carolina, metformin and tri-sprintec, on three prevalent aquatic species, snails (Ampullariidae), plants (Lemnoideae), and fleas (Daphnia magna). This experiment was done in two stages: phase one to study the effect of pharmaceuticals on the organisms and phase two to find the maximum threshold of pharmaceuticals affecting the lifespan of these species. It was hypothesized that when the dosage of pharmaceuticals increased, the Daphnia magna would experience increased heart rate, and the mortality and reproduction of these organisms would be negatively affected. This was achieved by placing the organisms in separate habitats, and three different doses of tri-sprintec and metformin were gradually added to their environments over three weeks. An ANOVA test of F(2,8)=15.88, p=0.002 for the heart rate of Daphnia magna and a linear regression t-test of R(3)=0.97, p<0.01 for the natural increase rate (NIR) of Daphnia magna showed that the pharmaceuticals did have a significant impact on these organisms. Furthermore, a linear regression t-test (R(3)=0.88, p<0.04) was run for Lemnoideae with metformin, and indicated that increased doses of pharmaceuticals did have a greater impact on the organisms. A brochure was circulated throughout neighborhoods to bring awareness about the best practices of disposing unused pharmaceuticals. This community outreach was a success and the researcher plans to continue conducting classes to the public and achieving the full potential of this experiment, thus reducing this environmental problem at the source. /