Title

The Inhibitory Effect Of Acesulfame Potassium On The Growth Of Rhodospirillum Rubrum

Author(s)

Nerielle Legaspi

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Environmental Science

Presentation Type

Non-Mentored

Written Paper Award

4th Place

Abstract

Acesulfame potassium is an artificial sweetener that can pass through the human body and wastewater treatment systems non-degraded. As a result, its presence in bodies of water is growing. It has even been proposed that it be used as a wastewater tracer in bodies of water, but it is mostly unknown how this artificial sweetener affects aquatic environments. The purpose of this experiment is to determine if the presence of acesulfame potassium would have any effect on aquatic environments by testing to see if it would inhibit the growth of Rhodospirillum rubrum, an environmental bacterium. It was hypothesized that as the concentration of acesulfame potassium gets larger in the bacterial cultures’ nutrient broth, the greater the inhibition of the growth of the R. rubrum. For experimentation, solutions with the acesulfame potassium concentrations of 0, 10, 20, 30, 40, and 50 μg/L were made. In the experiment, each nutrient broth solution with acesulfame potassium was placed into six test tubes along with R. rubrum. The test tubes were left for the bacteria to culture for ninety-six hours, during which 1 mL samples of each bacterial culture were taken every twenty-four hours and placed into cuvettes. The samples were analyzed using a SpectroVis to analyze the rate of growth of the bacterial cultures by measuring %T. This process was repeated four more times for a total of five trials. The data was statistically analyzed at alpha equal to 0.05 with a one-way ANOVA.

Location

Owens 210

Start Date

4-16-2016 9:45 AM

COinS
 
Apr 16th, 9:45 AM

The Inhibitory Effect Of Acesulfame Potassium On The Growth Of Rhodospirillum Rubrum

Owens 210

Acesulfame potassium is an artificial sweetener that can pass through the human body and wastewater treatment systems non-degraded. As a result, its presence in bodies of water is growing. It has even been proposed that it be used as a wastewater tracer in bodies of water, but it is mostly unknown how this artificial sweetener affects aquatic environments. The purpose of this experiment is to determine if the presence of acesulfame potassium would have any effect on aquatic environments by testing to see if it would inhibit the growth of Rhodospirillum rubrum, an environmental bacterium. It was hypothesized that as the concentration of acesulfame potassium gets larger in the bacterial cultures’ nutrient broth, the greater the inhibition of the growth of the R. rubrum. For experimentation, solutions with the acesulfame potassium concentrations of 0, 10, 20, 30, 40, and 50 μg/L were made. In the experiment, each nutrient broth solution with acesulfame potassium was placed into six test tubes along with R. rubrum. The test tubes were left for the bacteria to culture for ninety-six hours, during which 1 mL samples of each bacterial culture were taken every twenty-four hours and placed into cuvettes. The samples were analyzed using a SpectroVis to analyze the rate of growth of the bacterial cultures by measuring %T. This process was repeated four more times for a total of five trials. The data was statistically analyzed at alpha equal to 0.05 with a one-way ANOVA.