Title

Interleukin-2 And Interleukin-15 Differentially Impact The Transition Of Effector Cd8+ T Cells Into Effector- And Central- Memory Phenotype Cells

Author(s)

Brittany Shook

School Name

Governor's School for Science and Math

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: Dr. Rubinstein; Department of Surgery, Medical University of South Carolina

Abstract

With one in three women and one in two men diagnosed for cancer worldwide, cancer is becoming an increasing problem. More and more people are seeking unconventional treatment methods, including adoptive cell T cell transfer therapy. For this therapy to be most effective, effector-memory cytotoxic T cells are needed. In this experiment, cytokines are used to culture specific cell phenotypes. It was hypothesized that the addition of IL-2 will lead to effector memory phenotype T cells, while IL-15 will lead to more central memory phenotype cells. Using a male B6 mouse, the spleen was removed and the splenocytes were processed, the cells were stimulated using plate-bound anti-CD3 and soluble anti-CD28, and the cells were primed with cytokines IL-2 and IL-15. Flow cytometry was conducted at two time points: three and seven days after stimulation. The flow cytometry data showed that by day seven after activation, cells primed with IL-2 exhibited low levels of CD62L and was positive for the expression of CD44. Cells primed with IL-15, on the other hand, exhibited high levels of CD62L and were also positive for the expression of CD44. The data supported the hypothesis and it was concluded that IL-2 induced proliferation and differentiation of effector-memory phenotype cells, CD62L low CD44+, and IL-15 induced proliferation and differentiation of central-memory phenotype cells, CD62L high CD44+.

Location

Owens 202

Start Date

4-16-2016 9:00 AM

COinS
 
Apr 16th, 9:00 AM

Interleukin-2 And Interleukin-15 Differentially Impact The Transition Of Effector Cd8+ T Cells Into Effector- And Central- Memory Phenotype Cells

Owens 202

With one in three women and one in two men diagnosed for cancer worldwide, cancer is becoming an increasing problem. More and more people are seeking unconventional treatment methods, including adoptive cell T cell transfer therapy. For this therapy to be most effective, effector-memory cytotoxic T cells are needed. In this experiment, cytokines are used to culture specific cell phenotypes. It was hypothesized that the addition of IL-2 will lead to effector memory phenotype T cells, while IL-15 will lead to more central memory phenotype cells. Using a male B6 mouse, the spleen was removed and the splenocytes were processed, the cells were stimulated using plate-bound anti-CD3 and soluble anti-CD28, and the cells were primed with cytokines IL-2 and IL-15. Flow cytometry was conducted at two time points: three and seven days after stimulation. The flow cytometry data showed that by day seven after activation, cells primed with IL-2 exhibited low levels of CD62L and was positive for the expression of CD44. Cells primed with IL-15, on the other hand, exhibited high levels of CD62L and were also positive for the expression of CD44. The data supported the hypothesis and it was concluded that IL-2 induced proliferation and differentiation of effector-memory phenotype cells, CD62L low CD44+, and IL-15 induced proliferation and differentiation of central-memory phenotype cells, CD62L high CD44+.