3D Biofabrication of Cancer Cells and The Characterization of the Fisnar I&J7100

School Name

Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Engineering

Presentation Type

Mentored

Mentor

Mentor: Jorge Rodríguez-Dévora, Clemson University

Abstract

Up until now, scientists have used 2D models to test the effectiveness of cancer drugs. The issue with this method is that real, live tumors exist in three dimensions. In order to resolve this issue, a dispensing machine can be used to rapidly produce physiologically relevant cancer cells which can be aggregated to form tumors. The purpose of this experiment was to characterize the Fisnar I&J7100 dispensing machine so that it can be used to dispense 3D cell cultures and dispense drugs onto the cultures it creates. Water was used to test how pressure, dispense time, and viscosity affect the volume of liquid dispensed by the machine. Afterwards, 3T3 cells were dispensed and the relationship between cell count and dispense time was observed. The results showed that an increase in pressure and dispense time results in an increase in volume and that an increase in viscosity results in a decrease in volume. In the future, these results can be used to dispense specific numbers of cells into specific volumes of medium. Overall, the Fisnar I&J1700 proves to be a suitable device for cell printing. It provides a way to rapidly dispense cancer cells/aggregates to be used for high-throughput drug screening.

Location

Wall 223

Start Date

3-25-2017 11:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 11:15 AM

3D Biofabrication of Cancer Cells and The Characterization of the Fisnar I&J7100

Wall 223

Up until now, scientists have used 2D models to test the effectiveness of cancer drugs. The issue with this method is that real, live tumors exist in three dimensions. In order to resolve this issue, a dispensing machine can be used to rapidly produce physiologically relevant cancer cells which can be aggregated to form tumors. The purpose of this experiment was to characterize the Fisnar I&J7100 dispensing machine so that it can be used to dispense 3D cell cultures and dispense drugs onto the cultures it creates. Water was used to test how pressure, dispense time, and viscosity affect the volume of liquid dispensed by the machine. Afterwards, 3T3 cells were dispensed and the relationship between cell count and dispense time was observed. The results showed that an increase in pressure and dispense time results in an increase in volume and that an increase in viscosity results in a decrease in volume. In the future, these results can be used to dispense specific numbers of cells into specific volumes of medium. Overall, the Fisnar I&J1700 proves to be a suitable device for cell printing. It provides a way to rapidly dispense cancer cells/aggregates to be used for high-throughput drug screening.