Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters
Zhang, Y., Dawson, P.L., Hanks, T.W., Northcutt, J.K., Tzeng, T.R., Pennington, W.T. "Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters", Talanta, 2021, 221, 121482.
Membrane filters were coated with 10,12-pentacosadiynoic acid (PCDA) then polymerized on the filter for rapid bacterial detection and quantification. The polymerized PCDA (pPDCA)-coated filter changed color in response to Salmonella Typhimurium and Escherichia coli but not to Listeria innocua. The time required for color change of pPCDA-coated filters was determined by a visual panel. A simple linear regression model was generated to fit the observed data and was validated with goodness of fit analysis and residual analysis. The pPCDA-filter method estimated Salmonella Typhimurium populations of 8 to 3 log CFU ml−1 within 1.5–7.5 h, respectively.