Document Type

Article (Journal or Newsletter)

Scholarship Type

Faculty Scholarship, Student Scholarship

Publication Date



The purpose of this study was to develop a multi-layer diacetylene (PDA)-coated filter and to verify the proposed color response mechanism of PDA-coated filters to bacteria. Unlike other reports which immobilize PDA lipo- somes on solid support or create monolayer Langmuir films, multi-layer PDA-coated filters were generated by directly evaporating organic solvents. Different from incorporating functional headgroups or inserting phospho- lipids into PDA, we reported that bacterial growth can trigger the color change of PDA sensors without any mod- ification or phospholipid insertion. The mechanism that pH change from bacteria metabolites lead to color change of PDA filter was proposed and verified by carefully designed dextrose-free medium with phenol red. Fur- ther, culturing Salmonella Typhimurium, E. coli, L. innocua and M. luteus with PDA-coated filters on phenol red agar in absence of dextrose verified the potential of applying PDA-coated filter for bacterial detection, specifically amine-producing bacteria. Thus, PDA-coated filters may be a useful tool for food safety and shelf life applications.



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