# The Effect Of The Number Of Hours Horses Are Kept Stalled Per Day On The Number Of Strongyle Type Eggs And Larvae Present In Manure

## School Name

Heathwood Hall Episcopal School

## Grade Level

11th Grade

## Presentation Topic

Microbiology

## Presentation Type

Non-Mentored

## Written Paper Award

2nd Place

## Abstract

The purpose of this research project was to investigate the effect that the number of hours kept stalled per day had on the number of strongyle type eggs and larvae in equine manure samples. The independent variable was the number of hours horses were kept stalled per day. Horses were either stalled 0 hours per day (control group) or 8 hours per day. The dependent variable was the number of strongyle type eggs and larvae present in manure samples from these horses. It was hypothesized that the manure of horses stalled 8 hours per day would contain more strongyle type eggs and larvae than that of horses stalled 0 hours per day. The null hypothesis stated that there would be no difference between the two groups. The manure samples were collected, mixed with a sodium nitrate solution, then syringed into two chamber McMaster slides. The eggs and larvae were counted under a light microscope and the eggs per gram was calculated. A two tailed T-test (alpha=0.05) was completed to determine if there were statistically significant differences between the tested groups. The results suggested that these groups were not quite different enough for statistical significance to be considered.

## Recommended Citation

Tenhover, Julia, "The Effect Of The Number Of Hours Horses Are Kept Stalled Per Day On The Number Of Strongyle Type Eggs And Larvae Present In Manure" (2016). *South Carolina Junior Academy of Science*. 223.

https://scholarexchange.furman.edu/scjas/2016/all/223

## Location

Owens 110

## Start Date

4-16-2016 11:00 AM

The Effect Of The Number Of Hours Horses Are Kept Stalled Per Day On The Number Of Strongyle Type Eggs And Larvae Present In Manure

Owens 110

The purpose of this research project was to investigate the effect that the number of hours kept stalled per day had on the number of strongyle type eggs and larvae in equine manure samples. The independent variable was the number of hours horses were kept stalled per day. Horses were either stalled 0 hours per day (control group) or 8 hours per day. The dependent variable was the number of strongyle type eggs and larvae present in manure samples from these horses. It was hypothesized that the manure of horses stalled 8 hours per day would contain more strongyle type eggs and larvae than that of horses stalled 0 hours per day. The null hypothesis stated that there would be no difference between the two groups. The manure samples were collected, mixed with a sodium nitrate solution, then syringed into two chamber McMaster slides. The eggs and larvae were counted under a light microscope and the eggs per gram was calculated. A two tailed T-test (alpha=0.05) was completed to determine if there were statistically significant differences between the tested groups. The results suggested that these groups were not quite different enough for statistical significance to be considered.