The Inhibitory Effect Of Acesulfame Potassium On The Growth Of Rhodospirillum Rubrum
School Name
Spring Valley High School
Grade Level
11th Grade
Presentation Topic
Environmental Science
Presentation Type
Non-Mentored
Written Paper Award
4th Place
Abstract
Acesulfame potassium is an artificial sweetener that can pass through the human body and wastewater treatment systems non-degraded. As a result, its presence in bodies of water is growing. It has even been proposed that it be used as a wastewater tracer in bodies of water, but it is mostly unknown how this artificial sweetener affects aquatic environments. The purpose of this experiment is to determine if the presence of acesulfame potassium would have any effect on aquatic environments by testing to see if it would inhibit the growth of Rhodospirillum rubrum, an environmental bacterium. It was hypothesized that as the concentration of acesulfame potassium gets larger in the bacterial cultures’ nutrient broth, the greater the inhibition of the growth of the R. rubrum. For experimentation, solutions with the acesulfame potassium concentrations of 0, 10, 20, 30, 40, and 50 μg/L were made. In the experiment, each nutrient broth solution with acesulfame potassium was placed into six test tubes along with R. rubrum. The test tubes were left for the bacteria to culture for ninety-six hours, during which 1 mL samples of each bacterial culture were taken every twenty-four hours and placed into cuvettes. The samples were analyzed using a SpectroVis to analyze the rate of growth of the bacterial cultures by measuring %T. This process was repeated four more times for a total of five trials. The data was statistically analyzed at alpha equal to 0.05 with a one-way ANOVA.
Recommended Citation
Legaspi, Nerielle, "The Inhibitory Effect Of Acesulfame Potassium On The Growth Of Rhodospirillum Rubrum" (2016). South Carolina Junior Academy of Science. 243.
https://scholarexchange.furman.edu/scjas/2016/all/243
Location
Owens 210
Start Date
4-16-2016 9:45 AM
The Inhibitory Effect Of Acesulfame Potassium On The Growth Of Rhodospirillum Rubrum
Owens 210
Acesulfame potassium is an artificial sweetener that can pass through the human body and wastewater treatment systems non-degraded. As a result, its presence in bodies of water is growing. It has even been proposed that it be used as a wastewater tracer in bodies of water, but it is mostly unknown how this artificial sweetener affects aquatic environments. The purpose of this experiment is to determine if the presence of acesulfame potassium would have any effect on aquatic environments by testing to see if it would inhibit the growth of Rhodospirillum rubrum, an environmental bacterium. It was hypothesized that as the concentration of acesulfame potassium gets larger in the bacterial cultures’ nutrient broth, the greater the inhibition of the growth of the R. rubrum. For experimentation, solutions with the acesulfame potassium concentrations of 0, 10, 20, 30, 40, and 50 μg/L were made. In the experiment, each nutrient broth solution with acesulfame potassium was placed into six test tubes along with R. rubrum. The test tubes were left for the bacteria to culture for ninety-six hours, during which 1 mL samples of each bacterial culture were taken every twenty-four hours and placed into cuvettes. The samples were analyzed using a SpectroVis to analyze the rate of growth of the bacterial cultures by measuring %T. This process was repeated four more times for a total of five trials. The data was statistically analyzed at alpha equal to 0.05 with a one-way ANOVA.
