Effects of Ganoderma lucidum on Cell Migration

School Name

Dorman High School

Grade Level

11th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Mentor

Mentor: Neval Erturk, Converse College

Abstract

Ganoderma lucidum (reishi) is a fungal supplement known for its therapeutic properties. In this project, we investigated the effects of water extracts of reishi on cell migration of MCF-7 breast epithelial cell line. MCF-7 cells were cultured to 90% confluence in 12 well plates. Two scratch wounds per well were created by using a needle at the appropriate time point. Uniform wound formation was confirmed under the microscope. At the appropriate time point cells were fixed and stained. Wound were images taken under a 10X objective and the wound closure quantified using ImageJ software. We observed that at 12 and 18 hour treatment period reishi prevented wound closure significantly (p <.05). We recommend further evaluation of the effects of reishi on cell migration and to further explore the molecular mechanisms involved.

Location

Wall 209

Start Date

3-25-2017 10:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 25th, 10:45 AM

Effects of Ganoderma lucidum on Cell Migration

Wall 209

Ganoderma lucidum (reishi) is a fungal supplement known for its therapeutic properties. In this project, we investigated the effects of water extracts of reishi on cell migration of MCF-7 breast epithelial cell line. MCF-7 cells were cultured to 90% confluence in 12 well plates. Two scratch wounds per well were created by using a needle at the appropriate time point. Uniform wound formation was confirmed under the microscope. At the appropriate time point cells were fixed and stained. Wound were images taken under a 10X objective and the wound closure quantified using ImageJ software. We observed that at 12 and 18 hour treatment period reishi prevented wound closure significantly (p <.05). We recommend further evaluation of the effects of reishi on cell migration and to further explore the molecular mechanisms involved.