Establishing a Reference Model for Tailless’s (TLX) Expression Pattern and Developing a Tool to Manipulate the Expression Pattern of TLX using Blue Light
School Name
Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
In this study, cancer stem cells (CSCs) have been considered a potential therapeutic target for treating malignant tumors. Previous research has shown that the Tailless gene (Tlx) correlates with a neural stem cell's ability to self-replicate, but the exact interactions between Tlx’s expression pattern and cellular behavior are unknown. Two projects, the Suntag Site-Specific Knock-In (SKI) and the Blue Light Induction Model (BLIM), were conducted to establish a reference for Tlx’s default expression pattern and create a tool to manipulate Tlx’s expression pattern, respectively. While the SKI was unsuccessful, the plasmid transfection of EL222 and C(120)5 in BLIM was successful. However, the blue light induction has yet to be tested.
Recommended Citation
Bui, Thien-An, "Establishing a Reference Model for Tailless’s (TLX) Expression Pattern and Developing a Tool to Manipulate the Expression Pattern of TLX using Blue Light" (2018). South Carolina Junior Academy of Science. 13.
https://scholarexchange.furman.edu/scjas/2018/all/13
Location
Neville theater
Start Date
4-14-2018 9:30 AM
Presentation Format
Oral and Written
Establishing a Reference Model for Tailless’s (TLX) Expression Pattern and Developing a Tool to Manipulate the Expression Pattern of TLX using Blue Light
Neville theater
In this study, cancer stem cells (CSCs) have been considered a potential therapeutic target for treating malignant tumors. Previous research has shown that the Tailless gene (Tlx) correlates with a neural stem cell's ability to self-replicate, but the exact interactions between Tlx’s expression pattern and cellular behavior are unknown. Two projects, the Suntag Site-Specific Knock-In (SKI) and the Blue Light Induction Model (BLIM), were conducted to establish a reference for Tlx’s default expression pattern and create a tool to manipulate Tlx’s expression pattern, respectively. While the SKI was unsuccessful, the plasmid transfection of EL222 and C(120)5 in BLIM was successful. However, the blue light induction has yet to be tested.
