Title

Establishing a Reference Model for Tailless’s (TLX) Expression Pattern and Developing a Tool to Manipulate the Expression Pattern of TLX using Blue Light

Author(s)

Thien-An Bui, GSSM

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

In this study, cancer stem cells (CSCs) have been considered a potential therapeutic target for treating malignant tumors. Previous research has shown that the Tailless gene (Tlx) correlates with a neural stem cell's ability to self-replicate, but the exact interactions between Tlx’s expression pattern and cellular behavior are unknown. Two projects, the Suntag Site-Specific Knock-In (SKI) and the Blue Light Induction Model (BLIM), were conducted to establish a reference for Tlx’s default expression pattern and create a tool to manipulate Tlx’s expression pattern, respectively. While the SKI was unsuccessful, the plasmid transfection of EL222 and C(120)5 in BLIM was successful. However, the blue light induction has yet to be tested.

Location

Neville theater

Start Date

4-14-2018 9:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 9:30 AM

Establishing a Reference Model for Tailless’s (TLX) Expression Pattern and Developing a Tool to Manipulate the Expression Pattern of TLX using Blue Light

Neville theater

In this study, cancer stem cells (CSCs) have been considered a potential therapeutic target for treating malignant tumors. Previous research has shown that the Tailless gene (Tlx) correlates with a neural stem cell's ability to self-replicate, but the exact interactions between Tlx’s expression pattern and cellular behavior are unknown. Two projects, the Suntag Site-Specific Knock-In (SKI) and the Blue Light Induction Model (BLIM), were conducted to establish a reference for Tlx’s default expression pattern and create a tool to manipulate Tlx’s expression pattern, respectively. While the SKI was unsuccessful, the plasmid transfection of EL222 and C(120)5 in BLIM was successful. However, the blue light induction has yet to be tested.