The Effect of Irgasan of the embryonic development of Arbacia punctulata
School Name
Heathwood Hall Episcopal School
Grade Level
9th Grade
Presentation Topic
Zoology
Presentation Type
Non-Mentored
Abstract
This experiment demonstrates the effect of Irgasan on the embryonic development of sea urchins. The purpose of this experiment is to find if 0%, 0.01%, 0.1%, 1%, 1.7% Irgasan has a negative effect on the development of sea urchin embryos. Once the sea urchins arrived, they were injected with a .5% solution of potassium chloride that caused them to release their gametes. After the eggs and sperm were collected in separate cups, pipettes of egg and sperm were taken up and placed into petri dishes. The dishes were each filled with 25 ml of salt water that contained 0%, 0.01%, 0.1%, 1%, or 1.7% irgasan. After 17 hours the embryos were examined and data was collected on how far they had developed. When looking at the embryos under a microscope, it was clear (especially in the higher concentrations) that the chemical was affecting the embryos. They were discolored, misshapen, and had slow growth. Statistically it did not show an effect on the embryos because the averages of growth were not significantly different. In conclusion the embryos were negatively affected, but statistically it was not shown because of uneven levels of embryos in the petri dishes. This experiment demonstrates the effect of Irgasan on the embryonic development of sea urchins. The purpose of this experiment is to find if 0%, 0.01%, 0.1%, 1%, 1.7% Irgasan has a negative effect on the development of sea urchin embryos. Once the sea urchins arrived, they were injected with a .5% solution of potassium chloride that caused them to release their gametes. After the eggs and sperm were collected in separate cups, pipettes of egg and sperm were taken up and placed into petri dishes. The dishes were each filled with 25 ml of salt water that contained 0%, 0.01%, 0.1%, 1%, or 1.7% irgasan. After 17 hours the embryos were examined and data was collected on how far they had developed. When looking at the embryos under a microscope, it was clear (especially in the higher concentrations) that the chemical was affecting the embryos. They were discolored, misshapen, and had slow growth. Statistically it did not show an effect on the embryos because the averages of growth were not significantly different. In conclusion the embryos were negatively affected, but statistically it was not shown because of uneven levels of embryos in the petri dishes.
Recommended Citation
Barron, Catherine, "The Effect of Irgasan of the embryonic development of Arbacia punctulata" (2018). South Carolina Junior Academy of Science. 138.
https://scholarexchange.furman.edu/scjas/2018/all/138
Location
Neville 122
Start Date
4-14-2018 9:30 AM
Presentation Format
Oral and Written
The Effect of Irgasan of the embryonic development of Arbacia punctulata
Neville 122
This experiment demonstrates the effect of Irgasan on the embryonic development of sea urchins. The purpose of this experiment is to find if 0%, 0.01%, 0.1%, 1%, 1.7% Irgasan has a negative effect on the development of sea urchin embryos. Once the sea urchins arrived, they were injected with a .5% solution of potassium chloride that caused them to release their gametes. After the eggs and sperm were collected in separate cups, pipettes of egg and sperm were taken up and placed into petri dishes. The dishes were each filled with 25 ml of salt water that contained 0%, 0.01%, 0.1%, 1%, or 1.7% irgasan. After 17 hours the embryos were examined and data was collected on how far they had developed. When looking at the embryos under a microscope, it was clear (especially in the higher concentrations) that the chemical was affecting the embryos. They were discolored, misshapen, and had slow growth. Statistically it did not show an effect on the embryos because the averages of growth were not significantly different. In conclusion the embryos were negatively affected, but statistically it was not shown because of uneven levels of embryos in the petri dishes. This experiment demonstrates the effect of Irgasan on the embryonic development of sea urchins. The purpose of this experiment is to find if 0%, 0.01%, 0.1%, 1%, 1.7% Irgasan has a negative effect on the development of sea urchin embryos. Once the sea urchins arrived, they were injected with a .5% solution of potassium chloride that caused them to release their gametes. After the eggs and sperm were collected in separate cups, pipettes of egg and sperm were taken up and placed into petri dishes. The dishes were each filled with 25 ml of salt water that contained 0%, 0.01%, 0.1%, 1%, or 1.7% irgasan. After 17 hours the embryos were examined and data was collected on how far they had developed. When looking at the embryos under a microscope, it was clear (especially in the higher concentrations) that the chemical was affecting the embryos. They were discolored, misshapen, and had slow growth. Statistically it did not show an effect on the embryos because the averages of growth were not significantly different. In conclusion the embryos were negatively affected, but statistically it was not shown because of uneven levels of embryos in the petri dishes.
