Title

A comparison of the total flavonols in Daucus carota, Cyanococcus, Citrus sinensis, Citrus limon, Actinidia deliciosa, and Mangifera indica

Author(s)

Vikram Kumar, SVHS

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Chemistry

Presentation Type

Non-Mentored

Abstract

Degenerative diseases, as well as bacterial infections are caused by oxidative stress, and are a huge problem in the world. Antioxidants are needed to safely balance the harmful effects of free radicals without causing any damage to the body. Bioactive compounds which have powerful antioxidative abilities and can treat and prevent degenerative diseases. One of these many polyphenol derivatives is flavonols, which have are contained in many common edible plants. Examples are carrot, blueberry, lemon, kiwi, orange, and mango. Flavonols can be measured by creating an aqueous extract. 5 mL of the aqueous extract were mixed with 5 mL of Folin-Denis reagent and heated on a hot plate for a minute. The resulting solution was transferred into cuvettes and placed into a spectrovis that was pre-calibrated to a blank of Folin-Denis reagent. The extracts were measured for absorbance at a wavelength of 320 nm to determine relative flavonol levels. An ANOVA test at α = 0.05 (F(5)=1024,p < 0.05) was used to test the significance of the results. The ANOVA test returned a p-value of < 0.05, meaning the data was significant. This is because p < 0.05 is less than the alpha value, which means that means the null hypothesis that there was no difference between the extracts was rejected, as there were significant differences in the flavonol levels. The hypothesis that green colored extract would have the highest level of flavonols was not supported by the data, as blueberry has the highest level of flavonols.

Location

Neville 106

Start Date

4-14-2018 1:45 PM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 1:45 PM

A comparison of the total flavonols in Daucus carota, Cyanococcus, Citrus sinensis, Citrus limon, Actinidia deliciosa, and Mangifera indica

Neville 106

Degenerative diseases, as well as bacterial infections are caused by oxidative stress, and are a huge problem in the world. Antioxidants are needed to safely balance the harmful effects of free radicals without causing any damage to the body. Bioactive compounds which have powerful antioxidative abilities and can treat and prevent degenerative diseases. One of these many polyphenol derivatives is flavonols, which have are contained in many common edible plants. Examples are carrot, blueberry, lemon, kiwi, orange, and mango. Flavonols can be measured by creating an aqueous extract. 5 mL of the aqueous extract were mixed with 5 mL of Folin-Denis reagent and heated on a hot plate for a minute. The resulting solution was transferred into cuvettes and placed into a spectrovis that was pre-calibrated to a blank of Folin-Denis reagent. The extracts were measured for absorbance at a wavelength of 320 nm to determine relative flavonol levels. An ANOVA test at α = 0.05 (F(5)=1024,p < 0.05) was used to test the significance of the results. The ANOVA test returned a p-value of < 0.05, meaning the data was significant. This is because p < 0.05 is less than the alpha value, which means that means the null hypothesis that there was no difference between the extracts was rejected, as there were significant differences in the flavonol levels. The hypothesis that green colored extract would have the highest level of flavonols was not supported by the data, as blueberry has the highest level of flavonols.