Title

The effect of transition metals and organic acids on Escherichia Coli

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

Escherichia coli is a gram negative bacterium that is commonly found in the intestines of warm-blooded organisms such as the human species. The purpose of this study is to see if people could use transition metals and organic acids as an alternative for antibiotics. It was hypothesized that transition metals would inhibit the growth of E.coli more than organic acids. Twelve different variable groups were tested in three sets. The first set was made of two different organic acids groups, glutamic acid and aspartic acid . The second set was made up of two different transition metals:Iron (III) Sulfate and Copper Sulfate. The third set was made of a combination of both the transition metals and the organic acids. E. coli was grown in nutrient broth and exposed to the appropriate independent variable. Growth was measured using optical density. After 24 hours of growth, optical density was measured via absorbance using a SpectroVis at a 420nm. The data was analyzed using a multiple regression test. An ANOVA test was ran based off of the hypotheses H0: μ1 = μ2 = μ3 = μ4, and H1: at least one mean is different, at α = 0.05. The results of the ANOVA showed that at α = 0.05, F(8, 81) = 3.00220, p < 0.001. Based on this, it can be concluded that at least one mean was statistically significant from the others because p < 0.001 < α = 0.05. The variable that inhibited bacterial growth the most was the Iron (III) Sulfate.

Location

Neville 221

Start Date

4-14-2018 10:15 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 10:15 AM

The effect of transition metals and organic acids on Escherichia Coli

Neville 221

Escherichia coli is a gram negative bacterium that is commonly found in the intestines of warm-blooded organisms such as the human species. The purpose of this study is to see if people could use transition metals and organic acids as an alternative for antibiotics. It was hypothesized that transition metals would inhibit the growth of E.coli more than organic acids. Twelve different variable groups were tested in three sets. The first set was made of two different organic acids groups, glutamic acid and aspartic acid . The second set was made up of two different transition metals:Iron (III) Sulfate and Copper Sulfate. The third set was made of a combination of both the transition metals and the organic acids. E. coli was grown in nutrient broth and exposed to the appropriate independent variable. Growth was measured using optical density. After 24 hours of growth, optical density was measured via absorbance using a SpectroVis at a 420nm. The data was analyzed using a multiple regression test. An ANOVA test was ran based off of the hypotheses H0: μ1 = μ2 = μ3 = μ4, and H1: at least one mean is different, at α = 0.05. The results of the ANOVA showed that at α = 0.05, F(8, 81) = 3.00220, p < 0.001. Based on this, it can be concluded that at least one mean was statistically significant from the others because p < 0.001 < α = 0.05. The variable that inhibited bacterial growth the most was the Iron (III) Sulfate.