Title

The effect of developmental nutrition on ethanol addiction in Drosophila melanogaster

Author(s)

Dawn Nguyen, SVHS

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Abstract

Alcohol addiction and other substance abuse disorders have led to unhealthy life choices and mortality around the world. Thus, research has been conducted to study the correlation between epigenome and environmental factors in order to raise awareness and help for others. The purpose of this experiment was to analyze whether developmental nutrition affected ethanol addiction. It was hypothesized that the Drosophila melanogaster raised in a poor nutritional environment would consume the most ethanol. In order to test this hypothesis, the experiment consisted of raising the fruit flies in the developmental environments. Once the organisms became adults, a 0.1% ethanol and 5% sucrose mixture was introduced using a capillary feeding (CAFE) assay. This was used in order to study the ethanol consumption between each developmental treatment over two weeks. An average consumption rate of microliters per day was recorded. An ANOVA test [F(2,10) = 6.85, p = 0.013] showed that nutritional treatments impacted ethanol addiction. The Scheffé test found that the Fs of the rich treatment compared to the poor and control treatments to be greater than the critical value, 8.2. Overall, the cultures raised in rich developmental nutrition had the highest ethanol consumption.

Location

Neville 121

Start Date

4-14-2018 2:00 PM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 2:00 PM

The effect of developmental nutrition on ethanol addiction in Drosophila melanogaster

Neville 121

Alcohol addiction and other substance abuse disorders have led to unhealthy life choices and mortality around the world. Thus, research has been conducted to study the correlation between epigenome and environmental factors in order to raise awareness and help for others. The purpose of this experiment was to analyze whether developmental nutrition affected ethanol addiction. It was hypothesized that the Drosophila melanogaster raised in a poor nutritional environment would consume the most ethanol. In order to test this hypothesis, the experiment consisted of raising the fruit flies in the developmental environments. Once the organisms became adults, a 0.1% ethanol and 5% sucrose mixture was introduced using a capillary feeding (CAFE) assay. This was used in order to study the ethanol consumption between each developmental treatment over two weeks. An average consumption rate of microliters per day was recorded. An ANOVA test [F(2,10) = 6.85, p = 0.013] showed that nutritional treatments impacted ethanol addiction. The Scheffé test found that the Fs of the rich treatment compared to the poor and control treatments to be greater than the critical value, 8.2. Overall, the cultures raised in rich developmental nutrition had the highest ethanol consumption.