Title

LPAR Expression Levels During Chick Retinal Development

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

Lysophosphitidic acid (LPA) is a known extracellular signaling molecule which activates distinct receptors as encoded by LPAR genes. Recent work by Dr. Birgbauer has shown that LPA causes growth cone collapse in retinal ganglion cells (RGCs), suggesting that it is an important axon guidance molecule. We used the chick visual system as a model in order to study LPA receptor (LPAR) gene expression levels and patterns in the retina. We were looking at LPAR 4 specifically, because it is the least researched. To do this, we used a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) machine. Our results suggested to us that LPAR 4 tends to be expressed more in later stages of development. Discovering LPAR expression patterns in the retina could aid in identifying and treating congenital retinal defects such as albinism .

Location

Neville theater

Start Date

4-14-2018 9:00 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 9:00 AM

LPAR Expression Levels During Chick Retinal Development

Neville theater

Lysophosphitidic acid (LPA) is a known extracellular signaling molecule which activates distinct receptors as encoded by LPAR genes. Recent work by Dr. Birgbauer has shown that LPA causes growth cone collapse in retinal ganglion cells (RGCs), suggesting that it is an important axon guidance molecule. We used the chick visual system as a model in order to study LPA receptor (LPAR) gene expression levels and patterns in the retina. We were looking at LPAR 4 specifically, because it is the least researched. To do this, we used a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) machine. Our results suggested to us that LPAR 4 tends to be expressed more in later stages of development. Discovering LPAR expression patterns in the retina could aid in identifying and treating congenital retinal defects such as albinism .