Title

The effect of caffeine on the foraging behavior and reproduction of Drosophila melanogaster

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Abstract

The purpose of this experiment was to determine the effects of caffeine on the foraging behavior and reproduction of Drosophila melanogaster. It was hypothesized that if higher doses of caffeine were given, then the Drosophila would choose the lesser ideal option for their nutritional needs and the least amount of fertility would occur within that generation than Drosophila exposed to no caffeine. In testing the F1 generation for foraging behavior, the flies ingested varying doses (.001 g, .002 g, .005 g, .014 g, and .024 g of caffeine) and were given 15 minutes to forage for nutritionally substantial (banana) and nutritionally unsubstantial (sugar) food. In order to forage, two jars were placed into cut corners of a bag. The flies were transferred into the bag from the top of the Ziploc and foraged for either the banana or sugar. In testing the F2 generation, the mass of Drosophila population was calculated for the Drosophila whose F1 generations went to banana and to sugar. An ANOVA test [F (5.1148) =5.21, p < .001] on the effect of caffeine concentrations on the foraging behavior of Drosophila was significant. Both hypotheses for the reproduction of the F2 generation were supported. The mass of the F2 banana culture [r = -0.8530, r2 = 0.727686, p < 0.030807] was significantly correlated with caffeine concentration while the mass of the F2 sugar culture [r = 0.7949, r2 = 0.631944, p < 0.058758] was not. These results imply a high overall effect of caffeine on Drosophila.

Location

Neville 122

Start Date

4-14-2018 8:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 8:30 AM

The effect of caffeine on the foraging behavior and reproduction of Drosophila melanogaster

Neville 122

The purpose of this experiment was to determine the effects of caffeine on the foraging behavior and reproduction of Drosophila melanogaster. It was hypothesized that if higher doses of caffeine were given, then the Drosophila would choose the lesser ideal option for their nutritional needs and the least amount of fertility would occur within that generation than Drosophila exposed to no caffeine. In testing the F1 generation for foraging behavior, the flies ingested varying doses (.001 g, .002 g, .005 g, .014 g, and .024 g of caffeine) and were given 15 minutes to forage for nutritionally substantial (banana) and nutritionally unsubstantial (sugar) food. In order to forage, two jars were placed into cut corners of a bag. The flies were transferred into the bag from the top of the Ziploc and foraged for either the banana or sugar. In testing the F2 generation, the mass of Drosophila population was calculated for the Drosophila whose F1 generations went to banana and to sugar. An ANOVA test [F (5.1148) =5.21, p < .001] on the effect of caffeine concentrations on the foraging behavior of Drosophila was significant. Both hypotheses for the reproduction of the F2 generation were supported. The mass of the F2 banana culture [r = -0.8530, r2 = 0.727686, p < 0.030807] was significantly correlated with caffeine concentration while the mass of the F2 sugar culture [r = 0.7949, r2 = 0.631944, p < 0.058758] was not. These results imply a high overall effect of caffeine on Drosophila.