Isolation of Two Novel Bacteriophages With Host Mycobacterium smegmatis

Samantha Devapiriam, AMHS

Abstract

Currently, Tuberculosis (TB) is an expensive, intensive bacterial infection to treat in humans. The current methods for treating patients with TB consist largely of the use of antibiotics. Antibiotic treatments can be problematic, however, due to the many strains of TB that exist that are resistant to antibiotics. Howard Hughes Medical Institute started a Phage Discovery program designed for college students and scientists to find an alternative treatment to Tuberculosis. Bacteriophages have the potential to be a more efficient and cost-effective means to treat people afflicted with TB. The goal of the research presented herein is to discuss the procedures and benefits of discovering a bacteriophage through the Phage Discovery program. Out of the 32 environmental samples collected, 2 samples contained novel phages. One phage, named Lynn, is a lytic phage and the other phage, named Pascal, is determined to be a temperate phage. The phages were isolated on host M. smegmatis from enriched samples on a spot test. The DNA of phages Lynn and Pascal was isolated and will be purified and sent to the University of Pittsburgh for their genomes to be sequenced. Once the genomes are sequenced and annotated, they will be archived and made available to the scientific community for any research or studies done related to phages.

 
Apr 14th, 12:00 AM

Isolation of Two Novel Bacteriophages With Host Mycobacterium smegmatis

Currently, Tuberculosis (TB) is an expensive, intensive bacterial infection to treat in humans. The current methods for treating patients with TB consist largely of the use of antibiotics. Antibiotic treatments can be problematic, however, due to the many strains of TB that exist that are resistant to antibiotics. Howard Hughes Medical Institute started a Phage Discovery program designed for college students and scientists to find an alternative treatment to Tuberculosis. Bacteriophages have the potential to be a more efficient and cost-effective means to treat people afflicted with TB. The goal of the research presented herein is to discuss the procedures and benefits of discovering a bacteriophage through the Phage Discovery program. Out of the 32 environmental samples collected, 2 samples contained novel phages. One phage, named Lynn, is a lytic phage and the other phage, named Pascal, is determined to be a temperate phage. The phages were isolated on host M. smegmatis from enriched samples on a spot test. The DNA of phages Lynn and Pascal was isolated and will be purified and sent to the University of Pittsburgh for their genomes to be sequenced. Once the genomes are sequenced and annotated, they will be archived and made available to the scientific community for any research or studies done related to phages.