Identification of microRNAs as new blood biomarkers to predict breast cancer recurrence
School Name
Dutch Fork High School
Grade Level
10th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Oral Presentation Award
1st Place
Abstract
MicroRNAs (microRNAs) are short sequences of RNA (about 22 nucleotides) that are involved in the regulation of gene expression. Previous studies have suggested that a number of microRNAs are recognized as new biomarkers for cancers. The aim of this study is to identify specific microRNAs in serum, which may serve as potential diagnostic and prognostic biomarkers and therapeutic targets for breast cancer. Quantitative real-time PCR (qRT-PCR) array analyses of microRNAs in sera from four pairs of recurrent and nonrecurrent breast cancer patients were performed. Those differentially expressed microRNAs were verified in serum samples from 42 breast cancer patients. The prognostic values of the selected microRNAs were statistically analyzed, determined by the correlation between microRNA expression and tumor parameters. Three microRNAs (miR-134, miR-483-5p and miR-139-3p) in serum were identified as novel disease biomarkers and potential therapeutic targets for breast cancer. This is significant because current methods of detecting the recurrence of cancer, such as computed tomography (CT) scans and magnetic resonance imaging (MRI), are extremely expensive. If microRNAs in serum exosomes can be used for prognosis, then the process will become significantly more affordable.
Recommended Citation
chen, Lauren, "Identification of microRNAs as new blood biomarkers to predict breast cancer recurrence" (2018). South Carolina Junior Academy of Science. 242.
https://scholarexchange.furman.edu/scjas/2018/all/242
Location
Neville theater
Start Date
4-14-2018 11:00 AM
Presentation Format
Oral and Written
Identification of microRNAs as new blood biomarkers to predict breast cancer recurrence
Neville theater
MicroRNAs (microRNAs) are short sequences of RNA (about 22 nucleotides) that are involved in the regulation of gene expression. Previous studies have suggested that a number of microRNAs are recognized as new biomarkers for cancers. The aim of this study is to identify specific microRNAs in serum, which may serve as potential diagnostic and prognostic biomarkers and therapeutic targets for breast cancer. Quantitative real-time PCR (qRT-PCR) array analyses of microRNAs in sera from four pairs of recurrent and nonrecurrent breast cancer patients were performed. Those differentially expressed microRNAs were verified in serum samples from 42 breast cancer patients. The prognostic values of the selected microRNAs were statistically analyzed, determined by the correlation between microRNA expression and tumor parameters. Three microRNAs (miR-134, miR-483-5p and miR-139-3p) in serum were identified as novel disease biomarkers and potential therapeutic targets for breast cancer. This is significant because current methods of detecting the recurrence of cancer, such as computed tomography (CT) scans and magnetic resonance imaging (MRI), are extremely expensive. If microRNAs in serum exosomes can be used for prognosis, then the process will become significantly more affordable.
