Title

The Effects of Varying Colors of Light on Vascular Smooth Muscle Cells

Author(s)

Madeline Odom, HCC

School Name

Hamilton Career Center

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

Research shows that variations of the Opsin proteins are responsible for a higher response to different light sources.This has given scientists a control over cells and allowed the deactivating of overactive cells in lab mice. In this experiment, vascular smooth muscle cell line was started and subcultured until the appropriate number of cells were reached. Thirty six wells were required for the experiment. Cells were exposed to concentrations of 0%, 25%, 50% and 100% Opsin II proteins. The cells were exposed to the proteins over a period of 48 hours. Then, the first plate received blue light for ten minutes, the second plate received green, and the third plate received red light. Results were then found using the live/dead assay with the AMG microscope. The images showed the largest amount of growth with cells exposed to the 100% proteins and red light. The plate exposed to blue light had a majority of dead cells. Then, live cells were counted using the Biotek Synergy 4 machine. This machine showed quantitative data that supported the images from the AMG microscope.

Location

Neville theater

Start Date

4-14-2018 9:45 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 9:45 AM

The Effects of Varying Colors of Light on Vascular Smooth Muscle Cells

Neville theater

Research shows that variations of the Opsin proteins are responsible for a higher response to different light sources.This has given scientists a control over cells and allowed the deactivating of overactive cells in lab mice. In this experiment, vascular smooth muscle cell line was started and subcultured until the appropriate number of cells were reached. Thirty six wells were required for the experiment. Cells were exposed to concentrations of 0%, 25%, 50% and 100% Opsin II proteins. The cells were exposed to the proteins over a period of 48 hours. Then, the first plate received blue light for ten minutes, the second plate received green, and the third plate received red light. Results were then found using the live/dead assay with the AMG microscope. The images showed the largest amount of growth with cells exposed to the 100% proteins and red light. The plate exposed to blue light had a majority of dead cells. Then, live cells were counted using the Biotek Synergy 4 machine. This machine showed quantitative data that supported the images from the AMG microscope.