The Effects of Varying Colors of Light on Vascular Smooth Muscle Cells
School Name
Hamilton Career Center
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
Research shows that variations of the Opsin proteins are responsible for a higher response to different light sources.This has given scientists a control over cells and allowed the deactivating of overactive cells in lab mice. In this experiment, vascular smooth muscle cell line was started and subcultured until the appropriate number of cells were reached. Thirty six wells were required for the experiment. Cells were exposed to concentrations of 0%, 25%, 50% and 100% Opsin II proteins. The cells were exposed to the proteins over a period of 48 hours. Then, the first plate received blue light for ten minutes, the second plate received green, and the third plate received red light. Results were then found using the live/dead assay with the AMG microscope. The images showed the largest amount of growth with cells exposed to the 100% proteins and red light. The plate exposed to blue light had a majority of dead cells. Then, live cells were counted using the Biotek Synergy 4 machine. This machine showed quantitative data that supported the images from the AMG microscope.
Recommended Citation
Odom, Madeline, "The Effects of Varying Colors of Light on Vascular Smooth Muscle Cells" (2018). South Carolina Junior Academy of Science. 244.
https://scholarexchange.furman.edu/scjas/2018/all/244
Location
Neville theater
Start Date
4-14-2018 9:45 AM
Presentation Format
Oral and Written
The Effects of Varying Colors of Light on Vascular Smooth Muscle Cells
Neville theater
Research shows that variations of the Opsin proteins are responsible for a higher response to different light sources.This has given scientists a control over cells and allowed the deactivating of overactive cells in lab mice. In this experiment, vascular smooth muscle cell line was started and subcultured until the appropriate number of cells were reached. Thirty six wells were required for the experiment. Cells were exposed to concentrations of 0%, 25%, 50% and 100% Opsin II proteins. The cells were exposed to the proteins over a period of 48 hours. Then, the first plate received blue light for ten minutes, the second plate received green, and the third plate received red light. Results were then found using the live/dead assay with the AMG microscope. The images showed the largest amount of growth with cells exposed to the 100% proteins and red light. The plate exposed to blue light had a majority of dead cells. Then, live cells were counted using the Biotek Synergy 4 machine. This machine showed quantitative data that supported the images from the AMG microscope.
