Subcellular pH changes in correlation to Glutathione metabolism

Leah Hoffner, GSSM

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Microbiology

Presentation Type

Mentored

Abstract

The thiol redox balance is important for the function of prokaryotes and eukaryotes, playing a pivotal role in maintaining the proper function of particular proteins. These protein structures are stabilized by disulfide bonds. Glutathione (GSH) is an antioxidant that is believed to be essential for thiol redox control (Kumar, 2011) and shown to be a major cellular redox buffer (Grant and Posci, 1999 and 2004). pH changes may have a prominent influence on maintaining the ratio between reduced the glutathione and oxidized glutathione redox buffer (GSH: GSSG). Using S. cerevisiae (brewer’s yeast) as a model organism, we planned to find the correlation between GSH metabolism and pH changes during redox stress which would subsequently provide us insight about GSH trafficking and its metabolism. The period of research mentioned was unable to discover the correlation due to the death of the yeast cells.

Location

Neville 221

Start Date

4-14-2018 8:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 8:30 AM

Subcellular pH changes in correlation to Glutathione metabolism

Neville 221

The thiol redox balance is important for the function of prokaryotes and eukaryotes, playing a pivotal role in maintaining the proper function of particular proteins. These protein structures are stabilized by disulfide bonds. Glutathione (GSH) is an antioxidant that is believed to be essential for thiol redox control (Kumar, 2011) and shown to be a major cellular redox buffer (Grant and Posci, 1999 and 2004). pH changes may have a prominent influence on maintaining the ratio between reduced the glutathione and oxidized glutathione redox buffer (GSH: GSSG). Using S. cerevisiae (brewer’s yeast) as a model organism, we planned to find the correlation between GSH metabolism and pH changes during redox stress which would subsequently provide us insight about GSH trafficking and its metabolism. The period of research mentioned was unable to discover the correlation due to the death of the yeast cells.