Title

The Efficiency of Modified Temozolomide in Glioblastoma Cell Death

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Abstract

Glioblastomas are a neurological disease and its standard treatment is the chemotherapeutic drug, Temozolomide [TMZ]. However, the use of TMZ only prolongs the one-year survival rate by 6-10%. One way to increase the efficiency of TMZ is to modify the chemical structure. The aim of this research is to modify TMZ by either adding an Ester group [TMZ-E] or a carboxylic acid [TMZ-A] to the chemical structure. And to test these modified drugs along with unmodified TMZ on an oligodendrocyte cell line, LN-229. The cells were treated at a concentration of 0-50 μM for twenty-four hours, followed by a trypan blue assay to determine cell death. The results indicate that TMZ-E was more effective at killing glioblastoma cells than TMZ and TMZ-A. At 0.8 μM TMZ-E killed about 67.4% of the cancerous cells as to compared to unmodified TMZ which killed 30%. These various TMZs will also be tested on other cancer cell lines.

Location

Neville 322

Start Date

4-14-2018 11:30 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 11:30 AM

The Efficiency of Modified Temozolomide in Glioblastoma Cell Death

Neville 322

Glioblastomas are a neurological disease and its standard treatment is the chemotherapeutic drug, Temozolomide [TMZ]. However, the use of TMZ only prolongs the one-year survival rate by 6-10%. One way to increase the efficiency of TMZ is to modify the chemical structure. The aim of this research is to modify TMZ by either adding an Ester group [TMZ-E] or a carboxylic acid [TMZ-A] to the chemical structure. And to test these modified drugs along with unmodified TMZ on an oligodendrocyte cell line, LN-229. The cells were treated at a concentration of 0-50 μM for twenty-four hours, followed by a trypan blue assay to determine cell death. The results indicate that TMZ-E was more effective at killing glioblastoma cells than TMZ and TMZ-A. At 0.8 μM TMZ-E killed about 67.4% of the cancerous cells as to compared to unmodified TMZ which killed 30%. These various TMZs will also be tested on other cancer cell lines.