Title

Reducing Enolase Expression and Activity to Prevent Harmful Damage in Spinal Cord Injury

Author(s)

Jada Wilson, GSSM

School Name

Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Physiology and Health

Presentation Type

Mentored

Abstract

Approximately one in fifty thousand people suffer from spinal cord injury (SCI). This type of injury is extremely damaging and causes permanent neurological deficiencies. In this study, enolase activity was targeted by treating rats with the injury with ENOblock, Lipopolysaccharides (LPS), and both ENOblock/LPS. In previous studies, it was concluded that it is possible to improve/treat this condition by blocking neuron specific enolase (NSE). Neuron specific enolase is a protein associated with SCI that has been shown to increase the damage after a secondary injury that is due to the activation of inflammatory cascades and changes in NSE levels. It has also been shown that, after treatments of ENOblock (an enolase inhibitor) NSE levels decreased which could result in the development of a salutary treatment. In this study, the effects of ENOblock and LPS on induced rat cells was observed on several proteins. It was concluded that multiple treatments of ENOblock did not result in a significant increase in cell viability. However, the treatments appeared to be harmless to the cells. Further, there did not appear to be a significant effect on cell proteins. Keywords: spinal cord injury ᐧ enolase ᐧ ENOblock ᐧ Lipopolysaccharides

Location

Neville 322

Start Date

4-14-2018 11:00 AM

Presentation Format

Oral and Written

COinS
 
Apr 14th, 11:00 AM

Reducing Enolase Expression and Activity to Prevent Harmful Damage in Spinal Cord Injury

Neville 322

Approximately one in fifty thousand people suffer from spinal cord injury (SCI). This type of injury is extremely damaging and causes permanent neurological deficiencies. In this study, enolase activity was targeted by treating rats with the injury with ENOblock, Lipopolysaccharides (LPS), and both ENOblock/LPS. In previous studies, it was concluded that it is possible to improve/treat this condition by blocking neuron specific enolase (NSE). Neuron specific enolase is a protein associated with SCI that has been shown to increase the damage after a secondary injury that is due to the activation of inflammatory cascades and changes in NSE levels. It has also been shown that, after treatments of ENOblock (an enolase inhibitor) NSE levels decreased which could result in the development of a salutary treatment. In this study, the effects of ENOblock and LPS on induced rat cells was observed on several proteins. It was concluded that multiple treatments of ENOblock did not result in a significant increase in cell viability. However, the treatments appeared to be harmless to the cells. Further, there did not appear to be a significant effect on cell proteins. Keywords: spinal cord injury ᐧ enolase ᐧ ENOblock ᐧ Lipopolysaccharides