Histological Examination of Wheel Training on Diurnal Fluctuations In Mouse Skeletal Muscle
Abstract
Cancer-induced cachexia is characterized by the rapid decrease in body weight through the loss of muscle mass, and largely affects the mortality of cancer patients. Exercise has proven to increase quality of life scores and functionality of patients with cancer and may provide beneficial effects in the attenuation of cancer-induced muscle wasting. This preliminary study sought to evaluate the effects of an exercise regimen on skeletal muscle through histochemical methods. Healthy untrained male C57BL/6 mice were divided into three groups: 1) no wheel access controls, 2/3) 2 weeks of wheel access sacrificed after the light (7PM) and after the dark (7AM) cycle. The tibialis anterior (TA) was cryosectioned and used to evaluate myofiber changes in cross-section area (CSA), oxidative capacity by succinate dehydrogenase (SDH), and noncontractile tissue (surrogate maker for extracellular matrix) via wheat-germ agglutinin (WGA). Access to wheel provided statistically significant increase in total hindlimb weight, and extracellular matrix. Furthermore, wheel training increased overall CSA but not relative frequency of positive SDH fibers when compared to controls. While exercise is capable of inducing protein synthesis in untrained mice, we determine that training is necessary for increased hypertrophy.
Histological Examination of Wheel Training on Diurnal Fluctuations In Mouse Skeletal Muscle
Founders Hall 142 A
Cancer-induced cachexia is characterized by the rapid decrease in body weight through the loss of muscle mass, and largely affects the mortality of cancer patients. Exercise has proven to increase quality of life scores and functionality of patients with cancer and may provide beneficial effects in the attenuation of cancer-induced muscle wasting. This preliminary study sought to evaluate the effects of an exercise regimen on skeletal muscle through histochemical methods. Healthy untrained male C57BL/6 mice were divided into three groups: 1) no wheel access controls, 2/3) 2 weeks of wheel access sacrificed after the light (7PM) and after the dark (7AM) cycle. The tibialis anterior (TA) was cryosectioned and used to evaluate myofiber changes in cross-section area (CSA), oxidative capacity by succinate dehydrogenase (SDH), and noncontractile tissue (surrogate maker for extracellular matrix) via wheat-germ agglutinin (WGA). Access to wheel provided statistically significant increase in total hindlimb weight, and extracellular matrix. Furthermore, wheel training increased overall CSA but not relative frequency of positive SDH fibers when compared to controls. While exercise is capable of inducing protein synthesis in untrained mice, we determine that training is necessary for increased hypertrophy.
