Title

Identification of Novel Compounds that Inhibit Growth of Pseudomonas Aeruginosa by Targeting Penicillin-Binding Protein 3 (PBP3)

Author(s)

Daphne DangFollow

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Oral Presentation Award

1st Place

Abstract

My project will be addressing antimicrobial resistance in Pseudomonas aeruginosa, geared toward the hit-identification and structure-activity relationships. The targets of my investigation are the penicillin-binding proteins, and its purpose is to circumvent known resistance mechanisms. I will be using a model developed in the lab to select and screen potential inhibitors of penicillin-binding protein 3. Then, I will be characterizing the potency of these compounds through a series of biochemical and microbiological techniques such as SDS-PAGE and ITC. The purpose of this experiment is to identify novel compounds that could serve as enzyme inhibitors of Pseudomonas aeruginosa through targeting Pencillin Binding Proteins (PBPs) With three assays that include three different test compounds from the pyrrolidinedione class, I will discover that these compounds will inhibit Pseudomonas aeruginosa. DMSO will serve as our negative control as it does not inhibit P. aeruginosa. Tested compounds exhibit antimicrobial activity at high concentrations. Using a model developed in the lab, several potential inhibitors of PBP3 were selected and screened. The potency of tested compounds as PBP3 inhibitors is promising and show marginal antimicrobial activity against Pseudomonas aeruginosa reference strain PA14.

Location

Founders Hall 111 A

Start Date

3-30-2019 9:45 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 9:45 AM

Identification of Novel Compounds that Inhibit Growth of Pseudomonas Aeruginosa by Targeting Penicillin-Binding Protein 3 (PBP3)

Founders Hall 111 A

My project will be addressing antimicrobial resistance in Pseudomonas aeruginosa, geared toward the hit-identification and structure-activity relationships. The targets of my investigation are the penicillin-binding proteins, and its purpose is to circumvent known resistance mechanisms. I will be using a model developed in the lab to select and screen potential inhibitors of penicillin-binding protein 3. Then, I will be characterizing the potency of these compounds through a series of biochemical and microbiological techniques such as SDS-PAGE and ITC. The purpose of this experiment is to identify novel compounds that could serve as enzyme inhibitors of Pseudomonas aeruginosa through targeting Pencillin Binding Proteins (PBPs) With three assays that include three different test compounds from the pyrrolidinedione class, I will discover that these compounds will inhibit Pseudomonas aeruginosa. DMSO will serve as our negative control as it does not inhibit P. aeruginosa. Tested compounds exhibit antimicrobial activity at high concentrations. Using a model developed in the lab, several potential inhibitors of PBP3 were selected and screened. The potency of tested compounds as PBP3 inhibitors is promising and show marginal antimicrobial activity against Pseudomonas aeruginosa reference strain PA14.