Title

The Effect of Ethanol, N-Acetyl Cysteine, Vitamin C, and Glutathione on Behavior and Health In C. Elegans Exposed to Acrylamide

Author(s)

Khushi PatelFollow

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Oral Presentation Award

4th Place

Written Paper Award

2nd Place

Abstract

Over the past decades, the western diet has seen a rise in the number of fried food items that are consumed. This process of cooking can lead to the production of acrylamide, large amounts of which have been linked to multiple cancers, neurological problems, and fetal abnormalities (Kumar et al., 2018). The purpose of this experiment was to look at different types of therapies that could lessen the effects of acrylamide poisoning in C. elegans. It was hypothesized that if different therapies were added to C. elegans exposed to acrylamide, then glutathione would prove to be most successful. In this experiment, petri plates of agar and acrylamide were prepared with C. elegans. Additional petri dishes were prepared with therapies. The first set of plates, which had acrylamide present in them, were chunked into the respective plates of therapies. A data collection was conducted after one week where the number of nematode eggs, living nematodes, dead nematodes, and a phototaxis response were recorded. A One-Way ANOVA [F(3, 116)= 46.471, p<0.001] conducted on the number of dead nematodes resulted in a p-value less than 0.001. When compared to an -value of 0.05, this data was statistically significant. A One-Way ANOVA [F(3, 116)= 106.703, p<0.001] conducted on the phototaxis response data resulted in a p-value less than 0.001. When compared to an ⍺-value of 0.05, this data was statistically significant.

Location

Founders Hall 210 A.

Start Date

3-30-2019 10:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 10:15 AM

The Effect of Ethanol, N-Acetyl Cysteine, Vitamin C, and Glutathione on Behavior and Health In C. Elegans Exposed to Acrylamide

Founders Hall 210 A.

Over the past decades, the western diet has seen a rise in the number of fried food items that are consumed. This process of cooking can lead to the production of acrylamide, large amounts of which have been linked to multiple cancers, neurological problems, and fetal abnormalities (Kumar et al., 2018). The purpose of this experiment was to look at different types of therapies that could lessen the effects of acrylamide poisoning in C. elegans. It was hypothesized that if different therapies were added to C. elegans exposed to acrylamide, then glutathione would prove to be most successful. In this experiment, petri plates of agar and acrylamide were prepared with C. elegans. Additional petri dishes were prepared with therapies. The first set of plates, which had acrylamide present in them, were chunked into the respective plates of therapies. A data collection was conducted after one week where the number of nematode eggs, living nematodes, dead nematodes, and a phototaxis response were recorded. A One-Way ANOVA [F(3, 116)= 46.471, p<0.001] conducted on the number of dead nematodes resulted in a p-value less than 0.001. When compared to an -value of 0.05, this data was statistically significant. A One-Way ANOVA [F(3, 116)= 106.703, p<0.001] conducted on the phototaxis response data resulted in a p-value less than 0.001. When compared to an ⍺-value of 0.05, this data was statistically significant.