The Effect of Fiasma on Il-6 Secretion and Expression Stimulated By LPS and Palmitic Acid In Raw 264.7 Macrophages
School Name
South Carolina Governor's School for Science & Mathematics
Grade Level
12th Grade
Presentation Topic
Biochemistry
Presentation Type
Mentored
Abstract
Increased concentrations of palmitic acid (PA) and lipopolysaccharides (LPS) within the human body increase the likelihood for individuals to develop periodontitis. These substances appear in greater quantities in patients with diabetes and other metabolic syndromes. The purpose of this study was to discover the most potent inhibitor of IL-6 expression out of four different antidepressants known to be FIASMAs: amitriptyline (AMI), fluoxetine hydrochloride (FH), sertraline hydrochloride (SH), and paroxetine hydrochloride hemihydrate (PH). By culturing RAW264.7 mouse macrophages and running Enzyme-linked immunosorbent assays (ELISA) the expression of IL-6 can be quantified and analyzed. By using an antibody-antigen-antibody sandwich model, only the excess IL-6 was detected by the spectrometer indicating the potential damage that would be done to a human subject. The four FIASMAs were tested against LPS alone, PA alone, and LPS and PA together, the aim was to show the symbiotic relationship that the two substances have resulting in such serious cases of periodontitis. After analyzing the results, SH was the antidepressant with the greatest amount of inhibition. This will be a useful discovery to prescribe to diabetic patients, not to cure their diabetes associated periodontitis, but to decrease the severity of it or prevent it from becoming more severe.
Recommended Citation
Pope, Audrey, "The Effect of Fiasma on Il-6 Secretion and Expression Stimulated By LPS and Palmitic Acid In Raw 264.7 Macrophages" (2019). South Carolina Junior Academy of Science. 200.
https://scholarexchange.furman.edu/scjas/2019/all/200
Location
Founders Hall 111 A
Start Date
3-30-2019 10:15 AM
Presentation Format
Oral Only
Group Project
No
The Effect of Fiasma on Il-6 Secretion and Expression Stimulated By LPS and Palmitic Acid In Raw 264.7 Macrophages
Founders Hall 111 A
Increased concentrations of palmitic acid (PA) and lipopolysaccharides (LPS) within the human body increase the likelihood for individuals to develop periodontitis. These substances appear in greater quantities in patients with diabetes and other metabolic syndromes. The purpose of this study was to discover the most potent inhibitor of IL-6 expression out of four different antidepressants known to be FIASMAs: amitriptyline (AMI), fluoxetine hydrochloride (FH), sertraline hydrochloride (SH), and paroxetine hydrochloride hemihydrate (PH). By culturing RAW264.7 mouse macrophages and running Enzyme-linked immunosorbent assays (ELISA) the expression of IL-6 can be quantified and analyzed. By using an antibody-antigen-antibody sandwich model, only the excess IL-6 was detected by the spectrometer indicating the potential damage that would be done to a human subject. The four FIASMAs were tested against LPS alone, PA alone, and LPS and PA together, the aim was to show the symbiotic relationship that the two substances have resulting in such serious cases of periodontitis. After analyzing the results, SH was the antidepressant with the greatest amount of inhibition. This will be a useful discovery to prescribe to diabetic patients, not to cure their diabetes associated periodontitis, but to decrease the severity of it or prevent it from becoming more severe.
