Title

The Effect of Fiasma on Il-6 Secretion and Expression Stimulated By LPS and Palmitic Acid In Raw 264.7 Macrophages

Author(s)

Audrey PopeFollow

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Biochemistry

Presentation Type

Mentored

Abstract

Increased concentrations of palmitic acid (PA) and lipopolysaccharides (LPS) within the human body increase the likelihood for individuals to develop periodontitis. These substances appear in greater quantities in patients with diabetes and other metabolic syndromes. The purpose of this study was to discover the most potent inhibitor of IL-6 expression out of four different antidepressants known to be FIASMAs: amitriptyline (AMI), fluoxetine hydrochloride (FH), sertraline hydrochloride (SH), and paroxetine hydrochloride hemihydrate (PH). By culturing RAW264.7 mouse macrophages and running Enzyme-linked immunosorbent assays (ELISA) the expression of IL-6 can be quantified and analyzed. By using an antibody-antigen-antibody sandwich model, only the excess IL-6 was detected by the spectrometer indicating the potential damage that would be done to a human subject. The four FIASMAs were tested against LPS alone, PA alone, and LPS and PA together, the aim was to show the symbiotic relationship that the two substances have resulting in such serious cases of periodontitis. After analyzing the results, SH was the antidepressant with the greatest amount of inhibition. This will be a useful discovery to prescribe to diabetic patients, not to cure their diabetes associated periodontitis, but to decrease the severity of it or prevent it from becoming more severe.

Location

Founders Hall 111 A

Start Date

3-30-2019 10:15 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 10:15 AM

The Effect of Fiasma on Il-6 Secretion and Expression Stimulated By LPS and Palmitic Acid In Raw 264.7 Macrophages

Founders Hall 111 A

Increased concentrations of palmitic acid (PA) and lipopolysaccharides (LPS) within the human body increase the likelihood for individuals to develop periodontitis. These substances appear in greater quantities in patients with diabetes and other metabolic syndromes. The purpose of this study was to discover the most potent inhibitor of IL-6 expression out of four different antidepressants known to be FIASMAs: amitriptyline (AMI), fluoxetine hydrochloride (FH), sertraline hydrochloride (SH), and paroxetine hydrochloride hemihydrate (PH). By culturing RAW264.7 mouse macrophages and running Enzyme-linked immunosorbent assays (ELISA) the expression of IL-6 can be quantified and analyzed. By using an antibody-antigen-antibody sandwich model, only the excess IL-6 was detected by the spectrometer indicating the potential damage that would be done to a human subject. The four FIASMAs were tested against LPS alone, PA alone, and LPS and PA together, the aim was to show the symbiotic relationship that the two substances have resulting in such serious cases of periodontitis. After analyzing the results, SH was the antidepressant with the greatest amount of inhibition. This will be a useful discovery to prescribe to diabetic patients, not to cure their diabetes associated periodontitis, but to decrease the severity of it or prevent it from becoming more severe.