Title

The Effect of Prolonged Exposure of Copper Sulfate In Germicide on the Growth of Microbes

School Name

Heathwood Hall Episcopal School

Grade Level

10th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

The purpose of this experiment is to see if CuSO₄ in a cleaning product could continuously limit the growth on a surface after a period of time for use in a medical setting. If CuSO₄ is added into a hospital grade germicide, then the number of bacterial colonies after 8 hours will be less than a surface cleaned with germicide without CuSO₄. Four solutions with varying concentrations of CuSO₄ were used to clean a masked off portion of a school desk. The portion of the desk that was cleaned was swabbed eight hours later and used to inoculate 1 mL of luria broth. The broth was then incubated overnight then spread onto petri dishes and incubated overnight. The resulting colonies of bacteria were counted using the phone application ColonyCounter. After running a single factor ANOVA test the results found are statistically insignificant and do not support or refute the hypothesis because the results are inconclusive.

Location

Founders Hall 216 B

Start Date

3-30-2019 10:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 10:00 AM

The Effect of Prolonged Exposure of Copper Sulfate In Germicide on the Growth of Microbes

Founders Hall 216 B

The purpose of this experiment is to see if CuSO₄ in a cleaning product could continuously limit the growth on a surface after a period of time for use in a medical setting. If CuSO₄ is added into a hospital grade germicide, then the number of bacterial colonies after 8 hours will be less than a surface cleaned with germicide without CuSO₄. Four solutions with varying concentrations of CuSO₄ were used to clean a masked off portion of a school desk. The portion of the desk that was cleaned was swabbed eight hours later and used to inoculate 1 mL of luria broth. The broth was then incubated overnight then spread onto petri dishes and incubated overnight. The resulting colonies of bacteria were counted using the phone application ColonyCounter. After running a single factor ANOVA test the results found are statistically insignificant and do not support or refute the hypothesis because the results are inconclusive.