The Effect of Prolonged Exposure of Copper Sulfate In Germicide on the Growth of Microbes
School Name
Heathwood Hall Episcopal School
Grade Level
10th Grade
Presentation Topic
Microbiology
Presentation Type
Non-Mentored
Abstract
The purpose of this experiment is to see if CuSO₄ in a cleaning product could continuously limit the growth on a surface after a period of time for use in a medical setting. If CuSO₄ is added into a hospital grade germicide, then the number of bacterial colonies after 8 hours will be less than a surface cleaned with germicide without CuSO₄. Four solutions with varying concentrations of CuSO₄ were used to clean a masked off portion of a school desk. The portion of the desk that was cleaned was swabbed eight hours later and used to inoculate 1 mL of luria broth. The broth was then incubated overnight then spread onto petri dishes and incubated overnight. The resulting colonies of bacteria were counted using the phone application ColonyCounter. After running a single factor ANOVA test the results found are statistically insignificant and do not support or refute the hypothesis because the results are inconclusive.
Recommended Citation
Nwanagu, Johannamarie, "The Effect of Prolonged Exposure of Copper Sulfate In Germicide on the Growth of Microbes" (2019). South Carolina Junior Academy of Science. 223.
https://scholarexchange.furman.edu/scjas/2019/all/223
Location
Founders Hall 216 B
Start Date
3-30-2019 10:00 AM
Presentation Format
Oral and Written
Group Project
No
The Effect of Prolonged Exposure of Copper Sulfate In Germicide on the Growth of Microbes
Founders Hall 216 B
The purpose of this experiment is to see if CuSO₄ in a cleaning product could continuously limit the growth on a surface after a period of time for use in a medical setting. If CuSO₄ is added into a hospital grade germicide, then the number of bacterial colonies after 8 hours will be less than a surface cleaned with germicide without CuSO₄. Four solutions with varying concentrations of CuSO₄ were used to clean a masked off portion of a school desk. The portion of the desk that was cleaned was swabbed eight hours later and used to inoculate 1 mL of luria broth. The broth was then incubated overnight then spread onto petri dishes and incubated overnight. The resulting colonies of bacteria were counted using the phone application ColonyCounter. After running a single factor ANOVA test the results found are statistically insignificant and do not support or refute the hypothesis because the results are inconclusive.
