Title

The Effect of Sodium Fluoride, Turmeric, Ampicillin, and Xylitol Exposed to Sodium Lauryl Sulfate on the Inhibition of Streptococcus Mutans Biofilms

Author(s)

Anika NairFollow

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Oral Presentation Award

3rd Place

Abstract

Bacterial infections are a common problem in the field of dentistry. Bacteria exists as plaque and can form biofilms on the surface of teeth. Many recent dental diseases have rooted from the development of tooth biofilms. Finding a beneficial solution to inhibit the growth of these biofilms would reduce the risk of acquiring such diseases. The purpose of this experiment was to find the most effective treatment product which could successfully inhibit the most Streptococcus mutans biofilms. Knowing this would help others in the field of dentistry to incorporate such products in their daily practices in order to inhibit tooth biofilms and reduce the risk of dental diseases. It was hypothesized that if Streptococcus mutans were exposed to sodium lauryl sulfate and ampicillin, then the bacterial biofilms would absorb the most of this treatment, thus inhibiting their growth. Streptococcus mutans were grown in test tubes with different treatments added to them. The biofilms were then stained with crystal violet in order to detect the absorbance of the products on the biofilms. A one-way ANOVA test was run to test a difference in the means, and displayed a statistical difference between the means at α = 0.05, (F(4,143)=7.95957), p<0.05 when p<0.00001. A post-hoc Tukey test showed that the sodium lauryl sulfate and ampicillin treatment had a significantly higher absorbance mean compared to the other treatment means. Thus the hypothesis that using sodium lauryl sulfate and ampicillin in order to inhibit the highest amount of Streptococcus mutans biofilms was supported. It was concluded that using ampicillin and sodium lauryl sulfate in order to inhibit tooth biofilms is effective and could potentially be used in dental practices to reduce the development of dental diseases.

Location

Founders Hall 216 B

Start Date

3-30-2019 9:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 9:00 AM

The Effect of Sodium Fluoride, Turmeric, Ampicillin, and Xylitol Exposed to Sodium Lauryl Sulfate on the Inhibition of Streptococcus Mutans Biofilms

Founders Hall 216 B

Bacterial infections are a common problem in the field of dentistry. Bacteria exists as plaque and can form biofilms on the surface of teeth. Many recent dental diseases have rooted from the development of tooth biofilms. Finding a beneficial solution to inhibit the growth of these biofilms would reduce the risk of acquiring such diseases. The purpose of this experiment was to find the most effective treatment product which could successfully inhibit the most Streptococcus mutans biofilms. Knowing this would help others in the field of dentistry to incorporate such products in their daily practices in order to inhibit tooth biofilms and reduce the risk of dental diseases. It was hypothesized that if Streptococcus mutans were exposed to sodium lauryl sulfate and ampicillin, then the bacterial biofilms would absorb the most of this treatment, thus inhibiting their growth. Streptococcus mutans were grown in test tubes with different treatments added to them. The biofilms were then stained with crystal violet in order to detect the absorbance of the products on the biofilms. A one-way ANOVA test was run to test a difference in the means, and displayed a statistical difference between the means at α = 0.05, (F(4,143)=7.95957), p<0.05 when p<0.00001. A post-hoc Tukey test showed that the sodium lauryl sulfate and ampicillin treatment had a significantly higher absorbance mean compared to the other treatment means. Thus the hypothesis that using sodium lauryl sulfate and ampicillin in order to inhibit the highest amount of Streptococcus mutans biofilms was supported. It was concluded that using ampicillin and sodium lauryl sulfate in order to inhibit tooth biofilms is effective and could potentially be used in dental practices to reduce the development of dental diseases.