Title

The Effect of Sucralose Concentration on Mortality of Daphnia Magna

Author(s)

Andrew JuhnFollow

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Written Paper Award

3rd Place

Abstract

The artificial sweetener sucralose is able to pass through both the human body and sewage treatment facilities, making its way into the waterstream, and could be a potential threat to aquatic life. Daphnia magna, commonly known as the water flea, is an extremely common freshwater crustacean that plays a crucial role in the aquatic ecosystem, grazing on phytoplankton. The purpose of this experiment was to study the effects of hyperconcentrated amounts of sucralose on the mortality of Daphnia magna in order to see the impact of sucralose on the aquatic population. It was hypothesized that if the amount of sucralose in the water increased, then the mortality rates would increase. 150 Daphnia were set in 30 different observation bowls, with 5 in each bowl. There were three trials done, a control, a 100 μg trial, and a 1,000 mg trial. Each trial had 10 bowls. A One Way ANOVA (F(2.25)= 2.64, p = 0.091) was performed, which indicated that there was no significant difference between the treatments. The hypothesis was not supported and sucralose concentration did not have an impact on the mortality of Daphnia magna.

Location

Founders Hall 210 A

Start Date

3-30-2019 11:15 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 11:15 AM

The Effect of Sucralose Concentration on Mortality of Daphnia Magna

Founders Hall 210 A

The artificial sweetener sucralose is able to pass through both the human body and sewage treatment facilities, making its way into the waterstream, and could be a potential threat to aquatic life. Daphnia magna, commonly known as the water flea, is an extremely common freshwater crustacean that plays a crucial role in the aquatic ecosystem, grazing on phytoplankton. The purpose of this experiment was to study the effects of hyperconcentrated amounts of sucralose on the mortality of Daphnia magna in order to see the impact of sucralose on the aquatic population. It was hypothesized that if the amount of sucralose in the water increased, then the mortality rates would increase. 150 Daphnia were set in 30 different observation bowls, with 5 in each bowl. There were three trials done, a control, a 100 μg trial, and a 1,000 mg trial. Each trial had 10 bowls. A One Way ANOVA (F(2.25)= 2.64, p = 0.091) was performed, which indicated that there was no significant difference between the treatments. The hypothesis was not supported and sucralose concentration did not have an impact on the mortality of Daphnia magna.