The Effects of Acetylsalicylic Acid and Methylthioninium Chloride (MTC) on Mortality of Caenorhabditis Elegans with the Cl2006 Strain

Jay PatelFollow

School Name

spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Written Paper Award

3rd Place

Abstract

Alzheimer’s disease (AD), the most common form of dementia, is a neurodegenerative disorder of the brain that leads to memory loss. AD affects 5.3 million Americans and is the sixth leading cause of death in the United States. The purpose of this experiment was to test the efficiency of theorized tau and amyloid beta inhibitors on slowing down the effects of AD and to better understand the relationship between amyloid beta inhibitors and tau inhibitors. It was hypothesized that the group treated with acetylsalicylic acid and the group treated with MTC and acetylsalicylic acid would be the most effective in slowing down the neurodegeneration. C. elegans with the CL2006 strain was used to mimic AD. Before experimentation, 20 petri dishes were divided into quarters and cultured with C. elegans. Then, different treatments were added to the petri dishes. After five days, three areas from each trial were randomly chosen to calculate the average mortality rate. Percentages were the most ideal way to record the data because of the different amounts of C. elegans in each petri dish. An ANOVA test was conducted at an alpha value of 0.05. The p value was <0.001 which means there was statistical significance. A Tukey test was also conducted. There were honest statistical significance between the control and the group treated with MTC, the control and the group treated with MTC and acetylsalicylic acid, and the group treated with acetylsalicylic acid and the group treated with MTC and acetylsalicylic acid. The group treated with MTC and acetylsalicylic acid was the most effective at reducing the mortality rate of the C. elegans.

Location

Founders Hall 216 B

Start Date

3-30-2019 8:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 8:45 AM

The Effects of Acetylsalicylic Acid and Methylthioninium Chloride (MTC) on Mortality of Caenorhabditis Elegans with the Cl2006 Strain

Founders Hall 216 B

Alzheimer’s disease (AD), the most common form of dementia, is a neurodegenerative disorder of the brain that leads to memory loss. AD affects 5.3 million Americans and is the sixth leading cause of death in the United States. The purpose of this experiment was to test the efficiency of theorized tau and amyloid beta inhibitors on slowing down the effects of AD and to better understand the relationship between amyloid beta inhibitors and tau inhibitors. It was hypothesized that the group treated with acetylsalicylic acid and the group treated with MTC and acetylsalicylic acid would be the most effective in slowing down the neurodegeneration. C. elegans with the CL2006 strain was used to mimic AD. Before experimentation, 20 petri dishes were divided into quarters and cultured with C. elegans. Then, different treatments were added to the petri dishes. After five days, three areas from each trial were randomly chosen to calculate the average mortality rate. Percentages were the most ideal way to record the data because of the different amounts of C. elegans in each petri dish. An ANOVA test was conducted at an alpha value of 0.05. The p value was <0.001 which means there was statistical significance. A Tukey test was also conducted. There were honest statistical significance between the control and the group treated with MTC, the control and the group treated with MTC and acetylsalicylic acid, and the group treated with acetylsalicylic acid and the group treated with MTC and acetylsalicylic acid. The group treated with MTC and acetylsalicylic acid was the most effective at reducing the mortality rate of the C. elegans.