The Heart Rate of Daphnia Magna When Exposed to Ethanol and Resveratrol During Development

Author(s)

Khushi DaveFollow

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Abstract

Alcohol is a teratogen that can affect a person’s development in the womb. Resveratrol, found in wine, has been shown to help with cardiovascular diseases. The purpose of this study was to see the effect that the amount of alcohol had on heart rate as the Daphnia magna developed and if resveratrol could normalize that difference. It was hypothesized that as the amount of ethanol exposure increases, heart rate would decrease, and if resveratrol was introduced, then the heart rate would resemble control levels. This was done using Daphnia magna because of their transparent body that allows observation of the heart. Thirty D. magna were split into 4 levels, two of which were administered resveratrol. They were exposed to the solution for a total of 2 days, with heart rate observed and measured at 1, 24, and 48 hour marks. A repeated measures ANOVA was conducted to see if there was any interaction between time and treatment, and no interaction was found. This prompted a one-way ANOVA to compare the heart rate between the treatment, [F(4,303)=1.02, p=0.399]. This shows that there is no significant difference between heart rates. A steady increase in heart rate, however, was seen as time went on, and the mean heart rate was lower in treatments that included resveratrol. This shows that heart rate increases when exposed to ethanol for a long period of time, such as nine months, and resveratrol can lower that heart rate.

Location

Founders Hall 142 B

Start Date

3-30-2019 9:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 30th, 9:00 AM

The Heart Rate of Daphnia Magna When Exposed to Ethanol and Resveratrol During Development

Founders Hall 142 B

Alcohol is a teratogen that can affect a person’s development in the womb. Resveratrol, found in wine, has been shown to help with cardiovascular diseases. The purpose of this study was to see the effect that the amount of alcohol had on heart rate as the Daphnia magna developed and if resveratrol could normalize that difference. It was hypothesized that as the amount of ethanol exposure increases, heart rate would decrease, and if resveratrol was introduced, then the heart rate would resemble control levels. This was done using Daphnia magna because of their transparent body that allows observation of the heart. Thirty D. magna were split into 4 levels, two of which were administered resveratrol. They were exposed to the solution for a total of 2 days, with heart rate observed and measured at 1, 24, and 48 hour marks. A repeated measures ANOVA was conducted to see if there was any interaction between time and treatment, and no interaction was found. This prompted a one-way ANOVA to compare the heart rate between the treatment, [F(4,303)=1.02, p=0.399]. This shows that there is no significant difference between heart rates. A steady increase in heart rate, however, was seen as time went on, and the mean heart rate was lower in treatments that included resveratrol. This shows that heart rate increases when exposed to ethanol for a long period of time, such as nine months, and resveratrol can lower that heart rate.