Title

The Tracing of Mesothelial-Derived Cells

Author(s)

Brent HadleyFollow

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

The mesothelium is a layer of cells that surround the heart and multiple other organs that shows WT1 gene expression. My lab worked with WT1-Cre mice in order to trace the lineage of cells that are seen in the mesothelium at different embryo stages. My experiment observed the location that these cells existed and at what stage they were present. This experiment is necessary to ensure that experiments in the future and the past are authentic by verifying that the WT1-Cre expression can be considered specific in the heart at specific time points and areas. Knowing the location of this expression in earlier stages can also help to explain the effects that genetic modification can have on the body’s functions. The WT1 gene will be present in areas such as the liver and kidney at all the stages of the embryos in my case, E14.5, and E16.5, with constrictions of WT1 Cre+ that are associated with Rosa. This experiment involved the sectioning and staining of Cre+ mice embryos. They then went through a staining process which allowed us to view the presence of the desired genetic sequence. Once observing the locations of these genetic sequences, we determined the validity of the WT1-Cre expression specificity at different stages of the embryo.

Location

Founders Hall 114 A

Start Date

3-30-2019 11:00 AM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 11:00 AM

The Tracing of Mesothelial-Derived Cells

Founders Hall 114 A

The mesothelium is a layer of cells that surround the heart and multiple other organs that shows WT1 gene expression. My lab worked with WT1-Cre mice in order to trace the lineage of cells that are seen in the mesothelium at different embryo stages. My experiment observed the location that these cells existed and at what stage they were present. This experiment is necessary to ensure that experiments in the future and the past are authentic by verifying that the WT1-Cre expression can be considered specific in the heart at specific time points and areas. Knowing the location of this expression in earlier stages can also help to explain the effects that genetic modification can have on the body’s functions. The WT1 gene will be present in areas such as the liver and kidney at all the stages of the embryos in my case, E14.5, and E16.5, with constrictions of WT1 Cre+ that are associated with Rosa. This experiment involved the sectioning and staining of Cre+ mice embryos. They then went through a staining process which allowed us to view the presence of the desired genetic sequence. Once observing the locations of these genetic sequences, we determined the validity of the WT1-Cre expression specificity at different stages of the embryo.