Title

Comparing the Effect of TGFβ on Extracellular Matrix Gene Expression In Whole Skin and Dermal Fibroblasts

School Name

South Carolina Governor's School for Science & Mathematics

Grade Level

12th Grade

Presentation Topic

Cell and Molecular Biology

Presentation Type

Mentored

Abstract

Scleroderma, or Systemic Sclerosis (SSc), is a connective tissue disease that causes hardening / tightening of the skin and connective tissues. Scleroderma has a 50% 10-year mortality rate and fibrosis is responsible for 45% of deaths in the developed world. One of the staples of Scleroderma, fibrosis, is a leading cause of death worldwide but there are currently no FDA-approved agents capable of stopping or reversing fibrosis. When treating samples of whole skin and isolated skin fibroblasts with TGFβ(Transforming Growth Factor Beta), isolating RNA, synthesizing mRNA, then running quantitative real time polymerase chain reaction (qRTPCR), the increase in the strands of Collagen 1A1, Collagen 1A2, and Fibronectin in the extracellular matrix was expected to be greater. The induced response of the Collagen 1A1, Collagen 1A2, and Fibronectin in the extracellular matrix by TGFβ did have a similar trend of increasing in whole skin tissue and in primary skin fibroblasts. In the future, different concentrations of TGFβ should be tested in human skin to identify a concentration that generates a consistent response. The variability of the response in human skin from different individuals reflects susceptibility for developing fibrosis and/or different kinetics of response.

Location

Founders Hall 114 A

Start Date

3-30-2019 1:30 PM

Presentation Format

Oral Only

Group Project

No

COinS
 
Mar 30th, 1:30 PM

Comparing the Effect of TGFβ on Extracellular Matrix Gene Expression In Whole Skin and Dermal Fibroblasts

Founders Hall 114 A

Scleroderma, or Systemic Sclerosis (SSc), is a connective tissue disease that causes hardening / tightening of the skin and connective tissues. Scleroderma has a 50% 10-year mortality rate and fibrosis is responsible for 45% of deaths in the developed world. One of the staples of Scleroderma, fibrosis, is a leading cause of death worldwide but there are currently no FDA-approved agents capable of stopping or reversing fibrosis. When treating samples of whole skin and isolated skin fibroblasts with TGFβ(Transforming Growth Factor Beta), isolating RNA, synthesizing mRNA, then running quantitative real time polymerase chain reaction (qRTPCR), the increase in the strands of Collagen 1A1, Collagen 1A2, and Fibronectin in the extracellular matrix was expected to be greater. The induced response of the Collagen 1A1, Collagen 1A2, and Fibronectin in the extracellular matrix by TGFβ did have a similar trend of increasing in whole skin tissue and in primary skin fibroblasts. In the future, different concentrations of TGFβ should be tested in human skin to identify a concentration that generates a consistent response. The variability of the response in human skin from different individuals reflects susceptibility for developing fibrosis and/or different kinetics of response.