Title

The Effect of a Hydrochloric Acid Gradient on the Evaluation of Caenorhabditis elegans Nonseparable Judgment

Author(s)

Parth Desai

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Abstract

Microorganisms have been utilized to study the human nervous system for decades. However, the level of intelligence within the microorganism themselves has been minimally studied. The goal of this experiment was to determine the level of neural complexity in the microorganism, Caenorhabditis elegans. It was hypothesized that C. elegans would demonstrate a nonlinearly separable positive association with the higher and lower values of an HCl gradient and food, because they would be able to grasp the correlation between food and hydrochloric acid gradient learning. C. elegans were put into petri dishes with three distinct HCl concentrations. The C. elegans underwent three memory trials with 100μM and 300μM HCl; these trials taught them the association between 100μM and 300μM HCl and E. coli. The C. elegans were then put into petri dishes with all of the HCl concentrations to test their learned association. The position of the C. elegans were recorded after 1 minute and was the recorded data. Each C. elegans represented one trial. There was a minimum count of 30 C. elegans before experimentation began. Based on the results, C. elegans learned the positive association of 100μM and 300μM HCl with food and the negative association of 200μM HCl. A Pearsonś Chi-square test was used to determine the p-value. For all of the proof of concept and experimental trials the p-value was less than 0.025 thus statistically significant.

Location

Furman Hall 119

Start Date

3-28-2020 9:30 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 9:30 AM

The Effect of a Hydrochloric Acid Gradient on the Evaluation of Caenorhabditis elegans Nonseparable Judgment

Furman Hall 119

Microorganisms have been utilized to study the human nervous system for decades. However, the level of intelligence within the microorganism themselves has been minimally studied. The goal of this experiment was to determine the level of neural complexity in the microorganism, Caenorhabditis elegans. It was hypothesized that C. elegans would demonstrate a nonlinearly separable positive association with the higher and lower values of an HCl gradient and food, because they would be able to grasp the correlation between food and hydrochloric acid gradient learning. C. elegans were put into petri dishes with three distinct HCl concentrations. The C. elegans underwent three memory trials with 100μM and 300μM HCl; these trials taught them the association between 100μM and 300μM HCl and E. coli. The C. elegans were then put into petri dishes with all of the HCl concentrations to test their learned association. The position of the C. elegans were recorded after 1 minute and was the recorded data. Each C. elegans represented one trial. There was a minimum count of 30 C. elegans before experimentation began. Based on the results, C. elegans learned the positive association of 100μM and 300μM HCl with food and the negative association of 200μM HCl. A Pearsonś Chi-square test was used to determine the p-value. For all of the proof of concept and experimental trials the p-value was less than 0.025 thus statistically significant.