Title

The Effect of the Non Caloric Sweeteners Maltodextrin and Sucralose on the Consumption Rate and Behavior of Amoeba proteus

Author(s)

Caroline Brindel

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Zoology

Presentation Type

Non-Mentored

Abstract

Non-caloric sweeteners (NCS) are sugar substitutes that provide the same amount of sweetness as glucose without any calories. Their usage is increasing, and with this questions regarding safety are asked. The purpose of this study was to test the effects of consuming sucralose, maltodextrin, or both on the consumption rates and behavior of Amoeba proteus. This provides information on how these sweeteners are possibly attacked by amoeboid like white blood cells as well as possibly cause a weakness in the immune system. It was hypothesized that if Amoeba proteus were fed the noncaloric sweeteners maltodextrin, sucralose, or the two mixed, then those fed only maltodextrin would consume the most of the sweetener compared to the other groups, and if Amoeba proteus were fed the same noncaloric sweeteners, then those fed both would have the least behavior change among the groups. To test this groups of 100 amoeba were randomly divided with each group randomly assigned one of the sweeteners. One group was then randomly chosen, fed their assigned sweetener(s), and left for 24 hours. The amount consumed as well as the change in behavior was found. This was repeated with the remaining groups twice. Descriptive statistics were conducted supporting the hypothesis that the amoeba fed the maltodextrin consumed the most and that the amoeba fed the combined sweeteners would have the least change in behavior. Both ANOVAs, consumption and behavior data, showed significance F(3,7)=66.8994, p=0007 and F(3,7)=inf, p<0.001.

Location

Furman Hall 119

Start Date

3-28-2020 9:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 9:00 AM

The Effect of the Non Caloric Sweeteners Maltodextrin and Sucralose on the Consumption Rate and Behavior of Amoeba proteus

Furman Hall 119

Non-caloric sweeteners (NCS) are sugar substitutes that provide the same amount of sweetness as glucose without any calories. Their usage is increasing, and with this questions regarding safety are asked. The purpose of this study was to test the effects of consuming sucralose, maltodextrin, or both on the consumption rates and behavior of Amoeba proteus. This provides information on how these sweeteners are possibly attacked by amoeboid like white blood cells as well as possibly cause a weakness in the immune system. It was hypothesized that if Amoeba proteus were fed the noncaloric sweeteners maltodextrin, sucralose, or the two mixed, then those fed only maltodextrin would consume the most of the sweetener compared to the other groups, and if Amoeba proteus were fed the same noncaloric sweeteners, then those fed both would have the least behavior change among the groups. To test this groups of 100 amoeba were randomly divided with each group randomly assigned one of the sweeteners. One group was then randomly chosen, fed their assigned sweetener(s), and left for 24 hours. The amount consumed as well as the change in behavior was found. This was repeated with the remaining groups twice. Descriptive statistics were conducted supporting the hypothesis that the amoeba fed the maltodextrin consumed the most and that the amoeba fed the combined sweeteners would have the least change in behavior. Both ANOVAs, consumption and behavior data, showed significance F(3,7)=66.8994, p=0007 and F(3,7)=inf, p<0.001.