Title

The Effect of Natural Remedies for Epilepsy on the Recovery Time of Caenorhabditis elegans Being Electroshocked In Order to Induce a Seizure

Author(s)

Simran Tippabhatla

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Physiology and Health

Presentation Type

Non-Mentored

Abstract

Antiepileptic Drugs, or AEDs, are known to cause unwanted side effects such as nausea, fatigue, and vomiting. In order to prevent these side effects, the effectiveness of natural remedies, such as vitamins and herbs, has been considered. The use of natural remedies for epileptic treatment have not been completely researched, therefore providing the grounds for this experiment. Three groups of Caenorhabditis elegans were fed and raised using Escherichia coli. One group was left untreated, one group had Vitamin D extract on the surface of the agar plate, and the third group had chamomile extract on the surface of the agar plate. After 48 hours, M9 Saline Solution was poured into each plate and an electrical shock was run through it. Thirty worms from each group were observed to record how long it took for them to recover from the shock. After data from the three groups was analyzed, it was found that the control had an average recovery time of 204.532 seconds, the Vitamin D group had an average recovery time of 36.67 seconds, and the Chamomile group had an average recovery time of 84.76 seconds. A one way ANOVA test at alpha = 0.05 was conducted to conclude that the average recovery time of the groups were statistically significant. This is due to the fact that the F-statistic of 70.99 was greater than the critical f-value of 3.101. Additionally, p<0.05. A Tukey test determined that all three groups were significantly different from each other.

Location

Furman Hall 209

Start Date

3-28-2020 12:15 PM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 12:15 PM

The Effect of Natural Remedies for Epilepsy on the Recovery Time of Caenorhabditis elegans Being Electroshocked In Order to Induce a Seizure

Furman Hall 209

Antiepileptic Drugs, or AEDs, are known to cause unwanted side effects such as nausea, fatigue, and vomiting. In order to prevent these side effects, the effectiveness of natural remedies, such as vitamins and herbs, has been considered. The use of natural remedies for epileptic treatment have not been completely researched, therefore providing the grounds for this experiment. Three groups of Caenorhabditis elegans were fed and raised using Escherichia coli. One group was left untreated, one group had Vitamin D extract on the surface of the agar plate, and the third group had chamomile extract on the surface of the agar plate. After 48 hours, M9 Saline Solution was poured into each plate and an electrical shock was run through it. Thirty worms from each group were observed to record how long it took for them to recover from the shock. After data from the three groups was analyzed, it was found that the control had an average recovery time of 204.532 seconds, the Vitamin D group had an average recovery time of 36.67 seconds, and the Chamomile group had an average recovery time of 84.76 seconds. A one way ANOVA test at alpha = 0.05 was conducted to conclude that the average recovery time of the groups were statistically significant. This is due to the fact that the F-statistic of 70.99 was greater than the critical f-value of 3.101. Additionally, p<0.05. A Tukey test determined that all three groups were significantly different from each other.