Title

Investigating Alternative Synthesis and Impregnation Methods for Silver Nanoparticles

Author(s)

Yash Patel

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

As more traditional antibacterials are used, the number and frequency of multi-drug resistant bacteria increase. This rise in MDR bacteria is seen in many cases around the world, especially in developing countries. Many of these MDR bacterial strains originate and spread via hospitals and equipment. A possible solution is to sanitize the hospital equipment and surfaces frequently. Silver nanoparticles is a beneficial tool that can be used for disinfection of multidrug resistant bacteria. Due to its cost and negative environmental impacts, AgNPs have not been viable for mass production. AgNPs can be attached onto surfaces for a prolonged effect, thus maintaining sanitary environments. This study aims to investigate eco-friendly methods for AgNPs synthesis and impregnation. Two different variables will be tested, the stabilizing and reducing agent, NLE, OLE, and TSC, and the impregnation type, heat via iron (HI), and microwave irradiation (MI). It is hypothesized that the Azadirachta indica leaf extract used to create the AgNPs will produce a more effective solution compared to Olea europaea leaf extract, due to differences in molecular makeup, and that the MI will produce a stronger attachment between the disc and AgNPs, due to a higher energy. A Kruskal Wallis Test H, followed by an adjusted Dunn's post-hoc. The Kruskal-Wallis H test results, (H=56.72, 8 d.f., p=<0.01), showed that there were differences in the test groups, thus indicating to the alternative hypothesis. It is concluded that NLE along with HI are possible alternatives to traditional methods.

Location

Furman Hall 126

Start Date

3-28-2020 10:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 10:00 AM

Investigating Alternative Synthesis and Impregnation Methods for Silver Nanoparticles

Furman Hall 126

As more traditional antibacterials are used, the number and frequency of multi-drug resistant bacteria increase. This rise in MDR bacteria is seen in many cases around the world, especially in developing countries. Many of these MDR bacterial strains originate and spread via hospitals and equipment. A possible solution is to sanitize the hospital equipment and surfaces frequently. Silver nanoparticles is a beneficial tool that can be used for disinfection of multidrug resistant bacteria. Due to its cost and negative environmental impacts, AgNPs have not been viable for mass production. AgNPs can be attached onto surfaces for a prolonged effect, thus maintaining sanitary environments. This study aims to investigate eco-friendly methods for AgNPs synthesis and impregnation. Two different variables will be tested, the stabilizing and reducing agent, NLE, OLE, and TSC, and the impregnation type, heat via iron (HI), and microwave irradiation (MI). It is hypothesized that the Azadirachta indica leaf extract used to create the AgNPs will produce a more effective solution compared to Olea europaea leaf extract, due to differences in molecular makeup, and that the MI will produce a stronger attachment between the disc and AgNPs, due to a higher energy. A Kruskal Wallis Test H, followed by an adjusted Dunn's post-hoc. The Kruskal-Wallis H test results, (H=56.72, 8 d.f., p=<0.01), showed that there were differences in the test groups, thus indicating to the alternative hypothesis. It is concluded that NLE along with HI are possible alternatives to traditional methods.